Results 71 to 80 of about 1,860,209 (301)

New and simple optical method for in situ etch rate determination and endpoint detection

open access: yes, 2022
S.1474-1476Optical emission spectroscopy has been established as a simple method for simultaneous etch rate determination and endpoint detection in ion beam etching.
Heinrich, F.   +2 more
core   +1 more source

From junk to function — How weak selection in eukaryotes builds new parts and drives genomic complexity

open access: yesFEBS Letters, EarlyView.
How do genomes gain new functional parts? In eukaryotes, which tend to evolve under weak selection, much of the genome is junk. Palazzo and Qiu borrow the logic of Markov chains to show how non‐functional DNA becomes functional through the appearance of intermediate states, which arise due to epistasis, buffering, and biochemical messiness, allowing ...
Alexander F. Palazzo, Yi Qiu
wiley   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

open access: yesFEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge   +6 more
wiley   +1 more source

Development and validation of an alternative titration method for the determination of sulfate ion in indinavir sulfate

open access: yesQuímica Nova, 2005
A simple and rapid precipitation titration method was developed and validated to determine sulfate ion content in indinavir sulfate raw material.
Breno de Carvalho e Silva   +2 more
doaj   +1 more source

Building State and Security in Afghanistan and the Region

open access: yes, 2005
This report compiles materials related to the Liechtenstein Institute on Self-Determination¿s project, ¿Promoting Stability and Viability in Afghanistan and the Region,¿ which began in 2001 and ran through summer 2005, and considered the future of ...
Liechtenstein Institute on Self-Determination
core   +1 more source

L‐aspartate oxidase provides new insights into fumarate reduction in anaerobic darkness in Synechocystis sp. PCC6803

open access: yesFEBS Letters, EarlyView.
Synechocystis strains deficient in succinate dehydrogenase (SDH) secrete more succinate than the WT under dark anaerobic conditions, supporting that SDH then primarily acts as SDH, not as a fumarate reductase. L‐aspartate oxidase (Laspo) from Synechocystis is functional under anaerobic conditions, reducing fumarate to succinate.
Kateryna Kukil   +3 more
wiley   +1 more source

Determination of 50% endpoint titer using a simple formula

open access: yesWorld Journal of Virology, 2016
Two commonly used methods for calculating 50% endpoint using serial dilutions are Spearman-Karber method and Reed and Muench method. To understand/apply the above formulas, moderate statistical/mathematical skills are necessary. In this paper, a simple formula/method for calculating 50% endpoints has been proposed.
openaire   +2 more sources

A multiplex endpoint RT-PCR assay for quality assessment of RNA extracted from formalin-fixed paraffin-embedded tissues

open access: yesBMC Biotechnology, 2010
Background RNA extracted from formalin-fixed paraffin-embedded (FFPE) samples is chemically modified and degraded, which compromises its use in gene expression studies.
Dobrovic Alexander   +4 more
doaj   +1 more source

RNA‐related functions of BRCA2 in transcription, replication, and genome stability

open access: yesFEBS Letters, EarlyView.
Beyond its role in homologous recombination, BRCA2 regulates R‐loop homeostasis, DNA–RNA hybrid resolution, RNA polymerase II dynamics, and transcriptional activation. Disruption of any of these functions can contribute to genome instability, inflammatory signaling, and tumorigenesis. BRCA2 is a central tumor suppressor protein best known for its roles
Lucia Alvaro‐Aranda   +2 more
wiley   +1 more source

Determination of Trace Amounts of Hydrofluoric Acid in Non-Aqueous Solutions by the Coulometric Titration Method

open access: yesSensors, 2018
For monitoring of trace amounts of hydrofluoric acid in the organic fluorine chemical industry, a facile method for determination of the hydrofluoric acid in an ethanol solution of lithium chloride, by coulometric titration, was proposed.
Cairui Huang   +3 more
doaj   +1 more source

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