Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Critical Strain Energy Release Rate of Broken Ply Composite Laminates
, 1998 Ply breakage in laminated composites may be caused by impact load. Delamination usually precedes the failure of such broken ply laminates and it occurs at the interface of the broken and continuous plies.
B. Pradhan, Debabrata Chakraborty
core +1 more source
Lengthwise Crack Study of a Beam with Non-Linear Creep
Engineering TransactionsAn analytical study of the strain energy release rate for an inhomogeneous beam structure with a lengthwise crack subjected to non-linear creep is developed. The beam is inhomogeneous along its length.
Victor Iliev RIZOV
doaj +1 more source
Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10
FEBS Open Bio, EarlyView.Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee +6 more
wiley +1 more source
Temperature Effects on Critical Energy Release Rate for Aluminum and Titanium Alloys
This work investigates temperature’s effect on the critical energy release rate using damage mechanics material models and the element deletion method.
Teng Long +3 more
core +1 more source
Minimizing crack energy release rate by topology optimization
, 2018 Fatigue cracked primary aircraft structural parts that cannot be replaced need to be repaired by other means. A structurally efficient repair method is to use adhesively bonded patches as reinforcements.
Simonsson, Kjell, +17 more
core +1 more source
FEBS Open Bio, EarlyView.Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young +7 more
wiley +1 more source
A Refined Theory for Characterizing Adhesion of Elastic Coatings on Rigid Substrates Based on Pressurized Blister Test Methods: Closed-Form Solution and Energy Release Rate. [PDF]
Polymers (Basel), 2020 Lian YS +4 more
europepmc +1 more source
Acute caffeine treatment protects the developing retina from ischemia‐induced cell death
FEBS Open Bio, EarlyView.Caffeine reduces cell death in the developing retina under ischemia (OGD). This effect does not involve BDNF upregulation or antioxidant pathways (NRF2/VEGF). Neuroprotection occurs mainly through adenosine A2A receptor antagonism, decreasing glutamate release and excitotoxicity, highlighting caffeine's potential as an acute neuroprotective agent in ...
Amanda Alves Nascimento +6 more
wiley +1 more source
Effects of Starter Defect on Energy Release Rate of Three-Point End-Notch Flexure Tested Unidirectional Carbon Fiber Reinforced Polymer Composite. [PDF]
Polymers (Basel), 2020 Nor FM +5 more
europepmc +1 more source