Results 81 to 90 of about 369,296 (265)

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Kinematic Synthesis for Linkages with Velocity Targets

Journal of Advanced Mechanical Design, Systems, and Manufacturing, 2012
A gradient-based optimization method for designing linkages with velocity targets is described. Two theoretical application cases are established for four-bar linkage. In the first, a constant-velocity module is proposed for a point on the coupler.
Ana DE-JUAN, Ramon SANCIBRIAN, Pablo GARCÍA, Fernando VIADERO, Miguel IGLESIAS, Alfonso FERNÁNDEZ   +5 more
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Optimized bacteria are environmental prediction engines

Physical Review E, 2018
Experimentalists have observed phenotypic variability in isogenic bacteria populations. We explore the hypothesis that in fluctuating environments this variability is tuned to maximize a bacterium's expected log growth rate, potentially aided by epigenetic markers that store information about past environments.
Marzen, Sarah E, Crutchfield, James P
openaire   +7 more sources

Decarbonization Optimization of a Waste Heat Recovery System for Waste Tire Pyrolysis: Integrating AI-based Predictive Modeling and Thermodynamic Performance Validation

Journal of Applied Science and Engineering
20%–30% of total energy consumption in the global industrial sector is lost in the form of low-grade waste heat. The 100–200 °C exhaust waste heat generated by the pyrolysis process of waste tires has a remarkably high recovery potential, but its organic
Shih-Hsing Chang, Chin-Han Tsai, Chyan-Chyi Wu   +2 more
doaj   +1 more source

A Search Algorithm for Generating Alternative Process Plans in Flexible Manufacturing System

Journal of Advanced Mechanical Design, Systems, and Manufacturing, 2007
Capabilities and complexity of manufacturing systems are increasing and striving for an integrated manufacturing environment. Availability of alternative process plans is a key factor for integration of design, process planning and scheduling. This paper
Hossein TEHRANI, Nobuhiro SUGIMURA, Yoshitaka TANIMIZU, Koji IWAMURA   +3 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Identifying gene expression signatures for risk stratification of postoperative adjuvant chemotherapy in colorectal cancer

FEBS Open Bio, EarlyView.
A novel signature integrating genome‐wide analysis with clinical factors predicts recurrence in stage II colorectal cancer and enables a new risk stratification to guide postoperative adjuvant chemotherapy. Clinical risk stratification for postoperative recurrence in patients with pathological stage II (pStage II) colorectal cancer (CRC) is essential ...
Mayuko Otomo, Keisuke Okuno, Shuichi Watanabe, Sakiko Oba, Hiroyasu Kagawa, Masanori Tokunaga, Daisuke Ban, Yusuke Kinugasa   +7 more
wiley   +1 more source

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

Optimizing mechanical component design with population-based metaheuristics

Open Engineering
This study presents a comprehensive comparison of two population-based metaheuristic algorithms, differential evolution (DE) and particle swarm optimization (PSO), applied to the optimization of mechanical component design under both single-objective and
Nakkiew Warisa, Suriyanoi Sumanut, Nakkiew Wasawat, Wisittipanit Nuttachat   +3 more
doaj   +1 more source