Results 171 to 180 of about 308,220 (199)
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2017
Targeted mutagenesis is required to evaluate the function of DNA segments across the genome. In recent years the CRISPR/Cas9 technology has been widely used for functional genome studies and is partially replacing classical homologous recombination methods in different aspects.
Andrey Guillaume, Spielmann Malte
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Targeted mutagenesis is required to evaluate the function of DNA segments across the genome. In recent years the CRISPR/Cas9 technology has been widely used for functional genome studies and is partially replacing classical homologous recombination methods in different aspects.
Andrey Guillaume, Spielmann Malte
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Enhancer-associated RNAs as therapeutic targets
Expert Opinion on Biological Therapy, 2015Regulation of gene expression involves a variety of mechanisms driven by a complex regulatory network of factors. Control of transcription is an important step in gene expression regulation, which integrates the function of cis-acting and trans-acting elements.
Nicolas Léveillé +2 more
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Enhancer RNA: biogenesis, function, and regulation
Essays in Biochemistry, 2020Abstract Enhancers are noncoding DNA elements that are present upstream or downstream of a gene to control its spatial and temporal expression. Specific histone modifications, such as monomethylation on histone H3 lysine 4 (H3K4me1) and H3K27ac, have been widely used to assign enhancer regions in mammalian genomes.
Rong Ye, Changchang Cao, Yuanchao Xue
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RNA as performance-enhancers for dendritic cells
Expert Opinion on Biological Therapy, 2010Although studies have demonstrated that antigen-loaded dendritic cells (DC) elicit antigen-specific immune responses, the clinical benefit from DC-based cancer immunotherapy remains low. RNA, in the form of mRNA, has not only been used as a source of antigen but more recently as a way to stimulate DC to produce immunostimulatory molecules.
David, Boczkowski, Smita, Nair
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Visualization of Enhancer-Derived Noncoding RNA
2016Enhancers are principal regulators that allow spatiotemporal tissue-specific control of gene expression. While mounting evidence suggests that enhancer-derived long noncoding RNAs (long ncRNAs), including enhancer RNAs (eRNAs), are an important component of enhancer function, their expression has not been broadly analyzed at a single cell level via ...
Youtaro, Shibayama +2 more
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Enhanced RNA binding of dimerized aminoglycosides
Bioorganic & Medicinal Chemistry, 1999Aminoglycoside antibiotics have recently emerged as an intriguing family of RNA binding molecules and they became leading structures for the design of novel RNA ligands. The demystification of the aminoglycoside-RNA recognition phenomenon is required for the development of superior binders.
K, Michael, H, Wang, Y, Tor
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Enhancing Diagnosis Through RNA Sequencing
Clinics in Laboratory Medicine, 2020The diagnostic rate of comprehensive genomic sequencing remains only 25% to 30% due to the difficulty in interpreting variants of uncertain significance and noncoding mutations and in elucidating downstream effects of these and other genetic changes. Unlike DNA sequencing, RNA sequencing (RNAseq) reveals the functional consequence of genetic variation ...
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Enhancer RNAs: a class of long noncoding RNAs synthesized at enhancers.
Cold Spring Harbor perspectives in biology, 2015Recent studies have revealed that active enhancers are transcribed, producing a class of noncoding RNAs called enhancer RNAs (eRNAs). eRNAs are distinct from long noncoding RNAs (lncRNAs), but these two species of noncoding RNAs may share a similar role in the activation of mRNA transcription.
Tae-Kyung, Kim +2 more
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A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria
ACS Synthetic Biology, 2019A long-standing objective of metabolic engineering has been to exogenously increase the expression of target genes. In this research, we proposed the permanent RNA replication system using DNA as a template to store genetic information in bacteria. We selected Qβ phage as the RNA replication prototype and made many improvements to achieve target gene ...
Yi Yao +10 more
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An RNA Splicing Enhancer that Does Not Act by Looping
Angewandte Chemie International Edition, 2012Out of the loop: Do the proteins bound to an enhancer site on pre-mRNA interact directly with the splice site by diffusion (looping), as is generally accepted, or does the intervening RNA play a role? By inserting a PEG linker between an enhancer sequence and alternative splice sites, the interaction of these two elements can be studied.
Lewis, H +3 more
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