Results 61 to 70 of about 362,096 (260)

Design and analysis strategies for robust microbiome ageing research

FEBS Letters, EarlyView.
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik, İsmail Güderer, Yi Wang, Tayyaba Alvi, Prasoon Pandey, Handan Melike Dönertaş   +5 more
wiley   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

A conceptual framework for the temporal dynamics of spatial environmental heterogeneity–biodiversity relationships

Frontiers in Ecology and Evolution
Environmental heterogeneity is an important spatial characteristic, the loss of which is thought to reduce biodiversity by decreasing the availability of niche space.
Joseph Gent, Peter M. van Bodegom, Yali Si   +2 more
doaj   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Community Structure and Soil Environmental Drivers of Rhizosphere and Root Endophytic Microbiota of Polygonum divaricatum in a Temperate Grassland

Biology
Understanding the ecological drivers of plant-associated microbiota is essential for predicting grassland ecosystem resilience. This study aimed to characterize the community structure, functional potential, and soil environmental drivers of rhizosphere ...
Yubo Ren, Bo Zhang, Hui Jin, Xiaoyan Yang, Zhongxiang Xu, Yue Yuan, Cuiping Hua, Zuhua Yan, Bo Qin   +8 more
doaj   +1 more source

Discerning protein pools by selective staining with self‐labeling tags

FEBS Letters, EarlyView.
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley   +1 more source

Changes in Fish Taxonomic and Phylogenetic Diversity and Their Driving Factors in a Reservoir in the Karst Basin of Southwest China

Animals
Dam construction can significantly alter local habitat characteristics and the distribution patterns of aquatic organisms. However, the variations in the multidimensional diversity of fish assemblages in reservoirs, as well as the relative significance ...
Jialing Qiao, Yang Liu, Weiwei Yao, Hong Ma, Liang Yu, Qin Zhao, Lijian Ouyang   +6 more
doaj   +1 more source

Heterogeneous Nucleation and Growth of Nanoparticles at Environmental Interfaces

Accounts of Chemical Research, 2016
Mineral nucleation is a phase transformation of aqueous components to solids with an accompanying creation of new surfaces. In this evolutional, yet elusive, process, nuclei often form at environmental interfaces, which provide remarkably reactive sites for heterogeneous nucleation and growth.
Young-Shin, Jun, Doyoon, Kim, Chelsea W, Neil   +2 more
openaire   +2 more sources