Results 201 to 210 of about 1,493,909 (303)

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Conventional approaches to indicators and metrics undermine urban climate adaptation. [PDF]

NPJ Urban Sustain
Olazabal M, Mansur AV, Sahay S, Helmke-Long L, Granceri Bradaschia M, Villaverde A, Garmendia L, Dacosta Aboagye P, Sharifi A, Asamoah O, Mwangi P, Lewis W, Izaola B, Murtagh E, Feldman I.   +14 more
europepmc   +1 more source

Autophagosome marker, LC3, is released extracellularly via several distinct pathways

FEBS Open Bio, EarlyView.
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito, Masashi Arakawa, Koki Maeda, Eiji Morita   +3 more
wiley   +1 more source

Articles of Significant Interest in This Issue. [PDF]

Appl Environ Microbiol
europepmc   +1 more source

Environmental Variables in the Mexican Tropics and Their Relationship to Management and Welfare in Crossbreed Zebu Cattle. [PDF]

Animals (Basel)
Valdez MAD, Aguirre V, Rojas Hernández S, Olivares Pérez J, Pedernera M, Villa-Mancera A, Sarabia Salgado L, Olmedo-Juárez A, Quiroz Cardoso F, Cipriano Salazar M.   +9 more
europepmc   +1 more source

Navigating new norms: a systematic review of factors for the development of effective digital tools in higher education

FEBS Open Bio, EarlyView.
What factors make for an effective digital learning tool in Higher Education? This systematic review identifies elements of a digital tool that published examples reveal to be features of an engaging and impactful digital tool. A systematic literature search yielded 25 research papers for analysis.
Akmal Arzeman, Jessica Haines, Connie Pritchard, Stephen Rutherford, Nigel Francis   +4 more
wiley   +1 more source

Reassessing China's Regional Modernization Based on a Grey-Based Evaluation Framework and Spatial Disparity Analysis. [PDF]

Entropy (Basel)
Zhou W, Lin H, Zhang Z, Lin S.
europepmc   +1 more source

Hands‐on protocol for preparing water‐soluble fractions from agri‐food samples for NMR‐based metabolomics analysis

FEBS Open Bio, EarlyView.
This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso, Jaime Hiniesta‐Valero, Alejandra Guerra‐Castellano, Miguel A. De la Rosa, Irene Díaz‐Moreno   +4 more
wiley   +1 more source

The Impact of Green FinTech Promote Corporate Carbon Neutrality: Evidence from the Perspective of Financing Incentives and Scale Quality. [PDF]

Entropy (Basel)
Zhuang L, Wu C.
europepmc   +1 more source

Establishment of a coculture system for Porphyromonas gingivalis and head and neck squamous cell carcinoma using spheroid culture and LATS inhibition

FEBS Open Bio, EarlyView.
We established a spheroid coculture system enabling viable Porphyromonas gingivalis–HNSCC interactions under normoxic conditions. Inhibition of LATS1/2 maintains tumor cells in an undifferentiated state, which may promote spheroid growth and create a more permissive environment for bacterial persistence.
Yurika Nakajima, Shogo Okazaki, Muneaki Tamura, Shuichi Sato, Kenichi Imai   +4 more
wiley   +1 more source