Results 151 to 160 of about 930,563 (304)

Biological Populations as Indicators of Environmental Change - Volume 1

open access: yes, 1992
148 pages; available for download at the link below.This report, "Biological Populations as Indicators of Environmental Change - Volume 1" is a review of scientific literature on biological phenomena frequently noted in the popular press, to determine ...
United States Environmental Protection Agency Office of Policy, Planning and Evaluation
core  

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

open access: yesFEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel   +4 more
wiley   +1 more source

Mass selection in maize composites by means of selection indices

open access: yes, 1972
General considerationsA selection index may be applied to selection based either upon the performance of single plants or upon the performance of progenies.
Wolff, F., de
core  

STUDYING ENVIRONMENTAL AWARENESS IN THE SELECTION OF BUILDING MATERIALS

open access: yesJournal of Engineering and Sustainable Development, 2017
The main objective of the research is to highlight the extent of considering environmental impacts of building materials when making choices about the selection of building materials.
Entisar K. Al-Geelawee   +1 more
doaj  

Decreased cold‐sensing function of the transient receptor potential channel TRPM8 from tailed amphibians

open access: yesFEBS Open Bio, EarlyView.
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao   +3 more
wiley   +1 more source

Optimizing Voluntary Deforestation Policy in the Face of Adverse Selection and Costly Transfers

open access: yes
As part of international climate change policy, voluntary opt-in programs to reduce emissions in unregulated sectors or countries have spurred considerable discussion.
Kerr, Suzi, van Benthem, Arthur A.
core  

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

open access: yesFEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková   +3 more
wiley   +1 more source

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