Results 111 to 120 of about 360,705 (166)
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Enzymatic Activity of Desquamin
Experimental Cell Research, 1994We have previously described the properties of desquamin, a cell adhesion molecule in the stratum corneum with lectin-like properties specific for amino sugars. We report here that desquamin is also a trypsin-like serine proteinase. It degrades several chromogenic peptides with arginine in the P1 position, with greatest activity for the tissue ...
M M, Brysk +4 more
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D-Penicillamine and Enzymatic Activities
Enzyme, 2017The influence of incubation with D-penicillamine on pure enzyme preparations and on enzymatic activities of serum and skin homogenates was investigated. Three of the nine enzymatic activities studied underwent significant changes. Such effects of D-penicillamine must be taken into consideration if therapeutic or unwanted actions of this drug are to be ...
W, Raab, B, Gmeiner, P, Muckenhuber
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Non-enzymatic and enzymatic activation of mitomycin C: Identification of a unique cytosolic activity
International Journal of Cancer, 1996Mitomycin C (MMC), an alkylating anti-tumor agent, was activated by non-enzymatic and enzymatic mechanisms leading to DNA binding and adduct formation. However, it was enzymatically, not non-enzymatically, activated MMC which induced inter-strand DNA cross-linking, a major determinant of cell death.
P, Joseph, Y, Xu, A K, Jaiswal
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Sweetness and Enzymatic Activity of Lysozyme
Journal of Agricultural and Food Chemistry, 2001Hen egg lysozyme elicits a sweet taste sensation for human beings. Effects of reduction of disulfide bonds, heat treatment, and chemical modification of hen egg lysozyme on both sweetness and hydrolytic activity were investigated. Both the sweetness and enzymatic activities were lost when the intradisulfide linkage in a lysozyme molecule was reduced ...
T, Masuda, Y, Ueno, N, Kitabatake
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HPLC assay of enzymatic activities
Chromatographia, 1987Instead of traditional methods, high-performance liquid chromatography can be used for the determination of enzymatic activities. Assays can be performed once the reaction mixture have been injected in the HPLC apparatus. The elution of the samples is carried out with suitable buffers, and the product(s) formed and left oven substrate(s) are ...
PIETTA P, MAURI P, PACE M
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Presynaptic Neurotoxins with Enzymatic Activities
2008Toxins that alter neurotransmitter release from nerve terminals are of considerable scientific and clinical importance. Many advances were recently made in the understanding of their molecular mechanisms of action and use in human therapy. Here, we focus on presynaptic neurotoxins, which are very potent inhibitors of the neurotransmitter release ...
ROSSETTO, ORNELLA, MONTECUCCO, CESARE
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Measuring Enzymatic Activities with Nanopores
ChemBioChem, 2020AbstractNanopores have become powerful and versatile tools for measuring single molecules since their emergence in the mid‐1990s. They can be used to sense a wide variety of analytes including metal ions, small organic molecules, DNA/RNA, proteins, etc. to monitor chemical reactions, and to sequence DNA.
Yingying Sheng +3 more
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Pancreatin with High Enzymatic Activity*, †
Journal of the American Pharmaceutical Association (Scientific ed.), 1951A simple method of preparing pancreatin with high lipase content is outlined. An analysis, by 50 independent workers, of the pancreatin prepared by this method shows that it contains also over three times the amount of trypsin and of amylase required by the U. S. P. Thus it is an easily prepared “triple strength” pancreatin of high lipase content.
C W, BAUER, A J, VAZAKAS
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Enzymatic Activity of Human Carboxylesterases
Current Protocols in Toxicology, 2007AbstractThe carboxylesterases (CEs) are hydrolytic enzymes that metabolize xenobiotics that contain ester, thioester, or amide bonds. CEs are ubiquitously expressed but are found in highest concentration in membrane‐enriched fractions of the liver. This unit describes assays used to measure the enzymatic activity and tissue distribution of human CEs ...
Matthew K, Ross, Abdolsamad, Borazjani
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Triggering Enzymatic Activity with Force
Nano Letters, 2009Integrating single molecule force spectroscopy with fluorescence-based techniques allows the manipulation of an enzyme with a periodic stretching and relaxation protocol while simultaneously monitoring its catalytic activity. After releasing the stretching force we observe a higher probability for enzymatic activity at a time of 1.7 s.
Gumpp, H. +5 more
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