Results 221 to 230 of about 1,215,941 (358)

Progress in Molecular Imprinting-From Inhibition of Enzymatic Activity to Regulation of Cellular Pathways. [PDF]

Med Res Rev
Vodova M, Bezdekova J, Vaculovicova M.
europepmc   +1 more source

Diabetes‐induced vascular calcification is associated with low pyrophosphate and its oral supplementation prevents calcification in diabetic mice

FEBS Open Bio, EarlyView.
Induction of diabetes in three different mouse strains uniformly resulted in an increase in TNAP activity and a reduction in pyrophosphate (PPi) in the circulation. Inhibition of TNAP restored plasma PPi. Diabetes‐induced calcification in the media layer of the aorta was detected only in the Abcc6−/− strain, which is predisposed to ectopic ...
Krisztina Fülöp, Eszter Kozák, Natália Tőkési, Lilian Kocsis, Anikó Kelemen, Zsuzsanna Geszti, Adriána Kutás, Mariann Harangi, Ágnes Diószegi, Zsolt Rapi, József Balla, Olivier Le Saux, András Váradi, Viola Pomozi   +13 more
wiley   +1 more source

Enzymatic activity of PBP1B is required for growth rate-independent ppGpp-mediated resistance to PBP2 inhibitors in <i>E. coli</i>. [PDF]

J Bacteriol
Anderson SE, Mack IE, Levin PA.
europepmc   +1 more source

Pre and Postharvest Enzymatic Activity in Gulupa (Passiflora edulis Sims) Fruits from the Colombian Lower Montane Rain Forest

, 2014
Germán Franco, José Régulo Cartagena Valenzuela, Guillermo Antonio Correa Londoño, Benjamín Alberto Rojano, Ana María Piedrahíta Correa, Albert Ibarz, Víctor Falguera   +6 more
openalex   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Directed Evolution of Xylanase from <i>Dickeya dadantii</i> DCE-01 with Improved Enzymatic Activity. [PDF]

Polymers (Basel)
Wang R, Shi K, Zheng K, Yang Q, Xi G, Duan S, Cheng L.   +6 more
europepmc   +1 more source

Autophagosome marker, LC3, is released extracellularly via several distinct pathways

FEBS Open Bio, EarlyView.
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito, Masashi Arakawa, Koki Maeda, Eiji Morita   +3 more
wiley   +1 more source

Intrinsically disordered region of Clr4/Suv39 regulates its enzymatic activity and ensures heterochromatin spreading. [PDF]

Nucleic Acids Res
Nakamura R, Hayashi A, Nakagawa R, Yoshimura Y, Horikoshi N, Kurumizaka H, Nakayama JI.   +6 more
europepmc   +1 more source

Hands‐on protocol for preparing water‐soluble fractions from agri‐food samples for NMR‐based metabolomics analysis

FEBS Open Bio, EarlyView.
This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso, Jaime Hiniesta‐Valero, Alejandra Guerra‐Castellano, Miguel A. De la Rosa, Irene Díaz‐Moreno   +4 more
wiley   +1 more source

AFM-Based Monitoring of Enzymatic Activity of Individual Molecules of Cytochrome CYP102A1. [PDF]

Biosensors (Basel)
Ivanov YD, Bukharina NS, Shumov ID, Afonin ON, Tatur VY, Grudo AV, Archakov AI.   +6 more
europepmc   +1 more source