Results 31 to 40 of about 611,023 (294)

Monitoring of enzymatic reactions using capillary electrophoresis with conductivity detection [PDF]

, 2009
Capillary electrophoresis combined with contactless conductivity detection allows to separate and detect the ionic species, which are neither UV absorbing nor fluorescent.
Schuchert-Shi, Aiping
core   +1 more source

Optimization of one-step enzymatic hydrolysis conditions for two large brown algae and analysis of the antioxidant activity of the hydrolysis products

Electronic Journal of Biotechnology
Background: The industrial production of alginate lyase provides high-quality tool enzymes for the enzymatic hydrolysis of large brown algae. Establishing a seaweed enzymatic hydrolysis process that is simple to operate while offering both high enzymatic
Keqing Shi, Yitong Dong, Qi Wang, Ling Qin, Chen Zhong, Kefeng Xu, Mei Liu, Jie Sun   +7 more
doaj   +1 more source

Cellulose induced protein 1 (Cip1) from Trichoderma reesei enhances the enzymatic hydrolysis of pretreated lignocellulose

Microbial Cell Factories, 2021
Background Trichoderma reesei is currently the main strain for the commercial production of cellulase. Cellulose induced protein 1 (Cip1) is one of the most abundant proteins in extracellular proteins of T. reesei.
Hexue Jia, Wan Sun, Xuezhi Li, Jian Zhao
doaj   +1 more source

Glucose obtained from rice bran by ultrasound-assisted enzymatic hydrolysis

Ingeniería e Investigación, 2015
In this work ultrasound-assisted solid-state enzymatic hydrolysis of rice bran to obtain fermentable sugars was investigated. For this purpose, process variables such as temperature, enzyme concentration and moisture content were evaluated during the ...
Raquel Cristine Kuhn, Marcio Antonio Mazutti, Edson Luiz Foletto, Valéria Dal Prá, Eduardo Zimmermann, Matheus Souza, Vitória Segabinazzi Foletto, Tanisa Paula Silveira Maleski, Felipe Cavalheiro Lunelli, Pâmela Sfalcin   +9 more
doaj   +1 more source

Optimization of Extraction Process of Essential Oil from Bergamot Peel and Analysis of Its Components and Antioxidant Activity

Shipin gongye ke-ji, 2023
In this study, cellulase-assisted steam distillation was used to extract essential oil from bergamot peel. Based on the single factor analysis of enzymatic hydrolysis pH, enzymatic addition amount, enzymatic hydrolysis temperature and enzymatic ...
Yihe WANG, Yanli XIA, Xin ZHANG, Hang YANG, Guofu MEI, Bo YU, Jianming YI, Huiling XUE   +7 more
doaj   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Dynamic changes of peptidome and release of polysaccharide in sea cucumber (Apostichopus japonicus) hydrolysates depending on enzymatic hydrolysis approaches

Food Science and Human Wellness, 2022
Enzymatic hydrolysis has been widely used to produce bioactive hydrolysates from sea cucumber body wall. Here, inspired by the clarification of Apostichopus japonicus genome, we investigated the enzymatic hydrolysis of sea cucumber body wall by using the
Yanchao Wang, Yan Song, Yaoguang Chang, Yanyan Liu, Guangning Chen, Changhu Xue   +5 more
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Assessment the flavor of soybean meal hydrolyzed with Alcalase enzyme under different hydrolysis conditions by E-nose, E-tongue and HS-SPME-GC–MS

Food Chemistry: X, 2021
In the present study, E-nose, E-tongue, and headspace-solid phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC–MS) technology combined with Principal Component Analysis (PCA) were employed to evaluate the flavor characteristics of the
Zebin Weng, Lu Sun, Fang Wang, Xiaonan Sui, Yong Fang, Xiaozhi Tang, Xinchun Shen   +6 more
doaj   +1 more source