Results 51 to 60 of about 6,595,197 (312)

Summary of enzymatic properties of D2 variants.

, 2014
*>200 kDa for spore-inoculated nD2L.**Not confirmed by MS, but not a significant contribution to size shift.Summary of enzymatic properties of D2 variants.
Tuan-hua David Ho (624637), Su-May Yu (554727), Parastoo Azadi (223686), Mayumi Ishihara (455153), Aki Yoneda (624635), Hsion-Wen David Kuo (624636)   +5 more
core   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Screening, Identification, Whole Genome Analysis of Four Agarase-producing Vibrio Strains and Their Enzymatic Properties

Shipin gongye ke-ji
In order to expand the reservoir of highly active agarase-producing strains and to mine agarase-related gene pools, this study isolated strains from abalone viscera. Primary screening was conducted using a single carbon source medium combined with Lugo's
Liqun HUANG, Lianping ZHANG, Shuyi FAN, Liling YAO, Chentao YAN, Xulei ZHENG, Bin LIU, Ying HUANG   +7 more
doaj   +1 more source

Identification and Enzymatic Properties of Chitosanase Producing Marine Paenibacillus chitinolyticus Strain CLT08

Shipin gongye ke-ji, 2022
At present, chitosanase used to prepare chito-oligosaccharides has the disadvantage of low enzyme activity and poor acid stability. In this study, chitosanase-producing strain was screened from mud samples from Lianyungang Haizhou Bay, the highest ...
Tian LI, Zhiwen DING, Yaodong LIU, Rongjun ZHU, Wei ZHANG, Zhifa HUANG, Yaowei FANG, Shu LIU   +7 more
doaj   +1 more source

The microbiome in human skin aging

FEBS Letters, EarlyView.
Age‐related skin changes encompass the well‐known visible phenotypic alterations, together with microbiome dysbiosis and a series of molecular aging hallmarks. These hallmarks characterize not only a fully stablished aged phenotype but also the skin aging process itself.
Manuel Huerta Arana, Cornelia Wiegand, Omid Omrani, Mario Fabri   +3 more
wiley   +1 more source

Recent advances in functional studies of coronavirus NSP13 helicase and challenges in inhibitor development

Virulence
Coronavirus helicase NSP13 is essential for viral replication and transcription and is a promising target for broad-spectrum anti-coronavirus drugs due to its high sequence conservation and structural homology.
Yangxue Dai, Silan Li, Xue He, Keyu Lu, Junjie Tian, Yuanmou Huang, Deqin Wei, Xinyu He, Xiaolei Duan, Yang Liu, Bo Zhang   +10 more
doaj   +1 more source

Expression of β-glucanase Gene from Bacillus macerans in Escherichia coli

浙江大学学报. 农业与生命科学版, 2005
The β-glucanase gene from recombined cloning plasmid was subcloned into BamH Ⅰ and Xho Ⅰsite of expression plasmid pET-30a. The recombined expression plasmid was transformed into Escherichia coli strain BL21. The result of SDS-PAGE showed that about 25.0
LI Wei-fen, YAO Jiang-tao, HU Chun-xia, XU Zi-rong   +3 more
doaj   +1 more source

Characterization and Heterologous Expression of UDP-Glucose 4-Epimerase From a Hericium erinaceus Mutant with High Polysaccharide Production

Frontiers in Bioengineering and Biotechnology, 2021
Hericium erinaceus is an important medicinal fungus in traditional Chinese medicine because of its polysaccharides and other natural products. Compared terpenoids and polyketides, the analysis of synthetic pathway of polysaccharides is more difficult ...
Gen Zou, Juanbao Ren, Juanbao Ren, Di Wu, Henan Zhang, Ming Gong, Wen Li, Jingsong Zhang, Yan Yang   +8 more
doaj   +1 more source

Autophagy and mitophagy in pancreatic β‐cell homeostasis and their involvement in diabetes pathophysiology

FEBS Letters, EarlyView.
This review focuses on the role of autophagy and mitophagy in maintaining pancreatic β‐cell function and homeostasis. We discuss how genetic defects affecting these pathways contribute to the development of type 1, type 2, monogenic, and gestational diabetes. We further explore their potential as therapeutic targets. Created in BioRender.
Yunkyeong Lee, Mingming Tong, Anna L. Gloyn   +2 more
wiley   +1 more source

The Discovery, Enzymatic Characterization and Functional Analysis of a Newly Isolated Chitinase from Marine-Derived Fungus Aspergillus fumigatus df347

Marine Drugs, 2022
In order to discover a broad-specificity and high stability chitinase, a marine fungus, Aspergillus fumigatus df347, was identified in the sediments of mangrove wetlands in Qinzhou Bay, China. The chitinase gene (AfChi28) from A.
Ya-Li Wu, Sheng Wang, Deng-Feng Yang, Li-Yan Yang, Qing-Yan Wang, Jun Yu, Nan Li, Li-Xia Pan   +7 more
doaj   +1 more source