Background: Toxoplasmosis is a common parasitic infection of man, and reactivation of latent disease in HIV-infected patients can cause fatal encephalitis. Diagnosis depends on demonstration of parasite-specific antibodies in serum.
Sayan Bhattacharyya+2 more
doaj +1 more source
A combined efficient design for biomarker data subject to a limit of detection due to measuring instrument sensitivity [PDF]
Pooling specimens, a well-accepted sampling strategy in biomedical research, can be applied to reduce the cost of studying biomarkers. Even if the cost of a single assay is not a major restriction in evaluating biomarkers, pooling can be a powerful design that increases the efficiency of estimation based on data that is censored due to an instrument's ...
arxiv +1 more source
Time‐Controlled Dual Targeting to Program Systemic and Intercellular Transfer of Therapeutic Effects
Aspirin‐liposomes loaded onto monocytes enable inflammation‐triggered targeting and efficient hand‐over of aspirin to inflamed cells. Monocytes uptake a significant portion of aspirin‐liposomes, prolonging therapeutic action. This approach enhances anti‐inflammatory effects through intercellular transfer, demonstrating a translational strategy for ...
Seung Eun Yu+6 more
wiley +1 more source
Enzyme-linked immunosorbent assay for human plasma apolipoprotein B
A noncompetitive enzyme-linked immunosorbent assay (ELISA) has been developed for measuring total plasma apolipoprotein (apo) B using affinity purified polyclonal and monoclonal antibodies.
J M Ordovas+5 more
doaj
Instant determination of the potential biomarker heparan sulfate in human plasma by a mix-and-read fluorescence assay [PDF]
Heparan sulfate (HS) is a linear, polydisperse sulfated polysaccharide belonging to the glycosaminoglycan family. HS proteoglycans are ubiquitously found at the cell surface and extracellular matrix in animal species. HS is involved in the interaction with a wide variety of proteins and the regulation of many biological activities.
arxiv
Diagnosis of Zika Virus Infection by Peptide Array and Enzyme-Linked Immunosorbent Assay
Zika virus (ZIKV) is implicated in fetal stillbirth, microcephaly, intracranial calcifications, and ocular anomalies following vertical transmission from infected mothers.
N. Mishra+21 more
semanticscholar +1 more source
Semiconductor Electronic Label-Free Assay for Predictive Toxicology. [PDF]
While animal experimentations have spearheaded numerous breakthroughs in biomedicine, they also have spawned many logistical concerns in providing toxicity screening for copious new materials. Their prioritization is premised on performing cellular-level
Chui, Chi On+5 more
core +2 more sources
A multifunctional hydroxyapatite (HAp) coating integrated with silver‐gallium liquid metal nanoparticles (HAp‐Ag‐GaNPs) exhibits dual antibacterial and osteogenic properties. It effectively inhibits Gram‐positive and Gram‐negative bacteria, including resistant strains, while enhancing bone regeneration.
Ngoc Huu Nguyen+17 more
wiley +1 more source
IgG Adhesion on Hydrophobic Surfaces: Theory, Modelling, and Application to ELISA [PDF]
Enzyme-Linked ImmunoSorbent Assays (ELISA) are a range of widely used analytical methods whose implementation requires to build antibodies (IgG) thin films onto surfaces predominantly made of polystyrene. The high hydrophobicity of polystyrene ensures a spontaneous and strong adhesion of proteins allowing to easily build IgG monolayers. Since the ELISA
arxiv
The Effects of Statistical Multiplicity of Infection on Virus Quantification and Infectivity Assays [PDF]
Many biological assays are employed in virology to quantify parameters of interest. Two such classes of assays, virus quantification assays (VQA) and infectivity assays (IA), aim to estimate the number of viruses present in a solution, and the ability of a viral strain to successfully infect a host cell, respectively.
arxiv +1 more source