Results 71 to 80 of about 671,790 (405)

Biotin-Streptavidin Competition Mediates Sensitive Detection of Biomolecules in Enzyme Linked Immunosorbent Assay

open access: yesPLoS ONE, 2016
Enzyme Linked Immunosorbent Assay (ELISA) is the gold standard assay for detecting and identifying biomolecules using antibodies as the probe. Improving ELISA is crucial for detecting disease-causing agents and facilitating diagnosis at the early stages ...
T. LakshmiPriya, S. Gopinath, T. Tang
semanticscholar   +1 more source

Seroprevalence of toxocariasis in children aged 1-9 years in western Islamic Republic of Iran, 2003 [PDF]

open access: yes, 2007
We determined the seroprevalence of Toxocara canis infection in 544 children under 10 years randomly selected from urban and rural areas of Hamadan, An enzyme-linked immunosorbent assay was used for detection of antibodies to T.
Azimi, A., Fallah, M., Taherkhani, H.
core   +1 more source

An enzyme‐linked immunosorbent assay (ELISA)‐based activity assay for AMP‐activated protein kinase (AMPK)

open access: yesFEBS Open Bio, EarlyView.
Measuring AMP‐activated protein kinase (AMPK) activity in vitro is crucial for testing AMPK activators or inhibitors with therapeutic potential. Here, we report an enzyme‐linked immunosorbent assay (ELISA)‐based AMPK activity assay with simple steps and high sensitivity, which offers a simple, robust, and cost‐effective alternative to traditional ...
Trezze P. Nguyen, Shangze Lyu, Yang Liu
wiley   +1 more source

Pleural effusion caused by Trichinella spiralis infection: two case reports

open access: yesBMC Infectious Diseases, 2023
Background Trichinosis is a worldwide food-borne zoonotic parasitic disease, which is mainly obtained by ingesting undercooked meat containing infected larvae.
Zhen-zhen Pan   +3 more
doaj   +1 more source

Development of a New Monoclonal Antibody against Brevetoxins in Oyster Samples Based on the Indirect Competitive Enzyme-Linked Immunosorbent Assay

open access: yesFoods, 2021
The consumption of shellfish contaminated with brevetoxins, a family of ladder-frame polyether toxins formed during blooms of the marine dinoflagellate Karenia brevis, can cause neurotoxic poisoning, leading to gastroenteritis and neurotoxic effects.
Xiya Zhang   +8 more
doaj   +1 more source

Heterologous screening of hybridomas for the development of broad-specific monoclonal antibodies against deoxynivalenol and its analogues [PDF]

open access: yes, 2014
Hapten heterology was introduced into the steps of hybridoma selection for the development of monoclonal antibodies (MAbs) against deoxynivalenol (DON).
Bracke, Marc   +7 more
core   +1 more source

Development of an enzyme‑linked immunosorbent assay for camptothecin

open access: yesMolecular Medicine Reports, 2019
The use of camptothecin and its analogues has increased in clinical settings and in agriculture. Therefore, camptothecins and their derivatives, metabolites and degradation products are frequently found in the environment. Therefore, it is important to develop an ELISA for the quantification of camptothecins in human plasma, plants, animal tissues and ...
Li-Ting Yan   +6 more
openaire   +3 more sources

Whole‐Blood RNA Sequencing Profiling of Patients With Rheumatoid Arthritis Treated With Tofacitinib

open access: yesACR Open Rheumatology, EarlyView.
Objective Patients with rheumatoid arthritis (RA) often fail to respond to therapies, including JAK inhibitors (JAKi), and treatment allocation is made via a trial‐and‐error strategy. A comprehensive analysis of responses to JAKi, including tofacitinib, by RNA sequencing (RNAseq) would allow the discovery of transcriptomic markers with a two‐fold ...
Chiara Bellocchi   +11 more
wiley   +1 more source

Tacrolimus analysis: A comparison of different methods and matrices [PDF]

open access: yes, 1995
We determined the trough blood and plasma concentrations of tacrolimus from the day of transplantation through 30 days posttransplantation in four liver and four kidney transplant patients by three different methods.
Chao, J   +7 more
core   +1 more source

Activation of SIRT1 Reduces Renal Tubular Epithelial Cells Fibrosis in Hypoxia Through SIRT1‐FoxO1‐FoxO3‐Autophagy Pathway

open access: yesAdvanced Biology, EarlyView.
Hypoxia promotes the epithelial‐mesenchymal transition (EMT) of renal tubular epithelial cells via the SIRT1‐FoxO1‐FoxO3‐autophagy pathway, thereby resulting in the fibrosis of renal tubular epithelial cells. Activation of SIRT1 or induction of autophagy inhibits this process, alleviating hypoxia‐induced fibrosis.
Guangyu Wang   +6 more
wiley   +1 more source

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