Results 91 to 100 of about 6,233,293 (384)

Multiple strategies to improve the yield of chitinase a from Bacillus licheniformis in Pichia pastoris to obtain plant growth enhancer and GlcNAc

Microbial Cell Factories, 2020
Chitinase and chitin-oligosaccaride can be used in multiple field, so it is important to develop a high-yield chitinase producing strain. Here, a recombinant Pichia pastoris with 4 copies of ChiA gene from Bacillus licheniformis and co-expression of ...
Wen Song, Nuo Zhang, Mo Yang, Yuling Zhou, Nisha He, Guimin Zhang   +5 more
doaj   +1 more source

The iSplit GFP assay detects intracellular recombinant proteins in Bacillus subtilis

Microbial Cell Factories, 2021
Background Bacillus subtilis is one of the most important microorganisms for recombinant protein production. It possesses the GRAS (generally recognized as safe) status and a potent protein secretion capacity.
Patrick Lenz, Fabienne Hilgers, Alina Burmeister, Leonie Zimmermann, Kristina Volkenborn, Alexander Grünberger, Dietrich Kohlheyer, Thomas Drepper, Karl-Erich Jaeger, Andreas Knapp   +9 more
doaj   +1 more source

The effects of nitroxyl (HNO) on soluble guanylate cyclase activity: interactions at ferrous heme and cysteine thiols [PDF]

, 2009
It has been previously proposed that nitric oxide (NO) is the only biologically relevant nitrogen oxide capable of activating the enzyme soluble guanylate cyclase (sGC). However, recent reports implicate HNO as another possible activator of sGC.
Adrian J. Hobbs, Anderegg, Andrea J. Lee, Bari, Bartberger, Bermejo, Bonner, Brandwein, Burstyn, Craven, Craven, De Witt, DeMaster, Denninger, Derbyshire, DeRubertis, Dierks, Doyle, Drago, Dutton, Dutton, Ellis, Ellis, Farmer, Favaloro, Feelisch, Fukuto, Fukuto, Haddox, Halliwell, Hobbs, Hoshino, Ignarro, Ignarro, Ignarro, Ignarro, Immoos, Irvine, Irvine, Jon M. Fukuto, Judith N. Burstyn, Kamisaki, Katrina M. Miranda, Kim, Lin, Lopez, Maragos, Melisa M. Cherney, Miranda, Miranda, Miranda, Nestor E. Francoleon, Niederhoffer, Paolocci, Paolocci, Patrick J. Farmer, Pervitsky, Pino, Poulos, Rusche, S. Bruce King, Sayed, Scheidt, Schmidt, Serfass, Sha, Shafirovich, Shahidullah, Shen, Smith, Stasch, Stone, Stone, Sulc, Sulc, Thadani, Thomas W. Miller, Turk, Väänänen, Väänänen, Wanstall, Wayland, Wayland, Wolin, Wong, Yonetani   +85 more
core   +2 more sources

In vivo evidence for glycyl radical insertion into a catalytically inactive variant of pyruvate formate‐lyase

FEBS Letters, EarlyView.
Dimeric pyruvate formate‐lyase cleaves pyruvate using a radical‐based mechanism. G734 serves as a radical storage location, and the radical is transferred to the catalytic C419 residue. Mutation of the C418‐C419 pair causes loss of enzyme activity, but does not impede radical introduction onto G734. Therefore, cis‐ but not trans‐radical transfer occurs
Michelle Kammel, A. F. Volker Wagner, R. Gary Sawers   +2 more
wiley   +1 more source

Feruloyl esterase (FAE-1) sourced from a termite hindgut and GH10 xylanases synergy improves degradation of arabinoxylan

AMB Express, 2021
Cereal feedstocks have high arabinoxylan content as their main hemicellulose, which is linked to lignin by hydroxycinnamic acids such as ferulic acid.
Mpho S. Mafa, Samkelo Malgas, Brett I. Pletschke   +2 more
doaj   +1 more source

Metabolic Optimization by Enzyme-Enzyme and Enzyme-Cytoskeleton Associations [PDF]

, 2012
Probably enzymes are not dispersed in the cytoplasm, but are bound to each other and to specific cytoskeleton proteins. Associations result in substrate channeling from one enzyme to another. Multienzymatic complexes, or metabolons have been detected in glycolysis, the Krebs cycle and oxidative phosphorylation.
Adela Mújica, Antonio Peña, José G. Sampedro, Salvador Uribe-Carvajal, Natalia Chiquete-Félix, Monica Rosas-Lemus, Gisela Ruíz Granados, Daniela Araiza-Olivera   +7 more
openaire   +3 more sources

Alternative final steps in berberine biosynthesis in Coptis japonica cell cultures [PDF]

, 1988
In Coptis japonica cell cultures an alternative pathway has been discovered which leads from (S)-tetrahydrocolumbamine via (S)-canadine to berberine. The two enzymes involved have been partially purified.
Galneder, E., Rüffer, Martina, Tabata, M., Wanner, G., Zenk, Meinhart H.   +4 more
core   +1 more source

Evolutionary interplay between viruses and R‐loops

FEBS Letters, EarlyView.
Viruses interact with specialized nucleic acid structures called R‐loops to influence host transcription, epigenetic states, latency, and immune evasion. This Perspective examines the roles of R‐loops in viral replication, integration, and silencing, and how viruses co‐opt or avoid these structures.
Zsolt Karányi, Zita Képes, Zoltán Szabó, Eszter Csoma, Lóránt Székvölgyi   +4 more
wiley   +1 more source

GhWRKY1-like, a WRKY transcription factor, mediates drought tolerance in Arabidopsis via modulating ABA biosynthesis

BMC Plant Biology, 2021
Background Drought stress has great negative effects on the plant growth and development. The tolerance of plants to such abiotic stress is triggered by complicated and multilayered signaling pathways to restore cellular homeostasis and to promote ...
Qin Hu, Chuanwei Ao, Xiaorui Wang, Yanfei Wu, Xuezhu Du   +4 more
doaj   +1 more source

Enzyme immobilisation in biocatalysis: why, what and how.

Chemical Society Reviews, 2013
In this tutorial review, an overview of the why, what and how of enzyme immobilisation for use in biocatalysis is presented. The importance of biocatalysis in the context of green and sustainable chemicals manufacture is discussed and the necessity for ...
R. Sheldon, S. van Pelt
semanticscholar   +1 more source