Results 91 to 100 of about 6,346,353 (348)

4‐nitrobenzoate inhibits 4‐hydroxybenzoate polyprenyltransferase in malaria parasites and enhances atovaquone efficacy

FEBS Letters, EarlyView.
Atovaquone is an antimalarial requiring potentiation for sufficient efficacy. We pursued strategies to enhance its activity, showing that 4‐nitrobenzoate inhibits 4‐hydroxybenzoate polyprenyltransferase, decreasing ubiquinone biosynthesis. Since atovaquone competes with ubiquinol in mitochondria, 4‐nitrobenzoate facilitates its action, potentiating ...
Ignasi Bofill Verdaguer, Matheus Felipe Santos, Maurício Mazzine Filho, Gabriela Oliveira Castro, Agustín Hernández, Manoel Aparecido Peres, Alejandro Miguel Katzin, Marcell Crispim   +7 more
wiley   +1 more source

Fhl1p protein, a positive transcription factor in Pichia pastoris, enhances the expression of recombinant proteins

Microbial Cell Factories, 2019
Background The methylotrophic yeast Pichia pastoris is well-known for the production of a broad spectrum of functional types of heterologous proteins including enzymes, antigens, engineered antibody fragments, and next gen protein scaffolds and many ...
Xueyun Zheng, Yimin Zhang, Xinying Zhang, Cheng Li, Xiaoxiao Liu, Ying Lin, Shuli Liang   +6 more
doaj   +1 more source

Automating functional enzyme screening & characterization [PDF]

, 2018
This work has been presented in the 10th IWBDA workshop.Microfluidics continue to gain traction as an inexpensive alternative to standard multi-well plate-based, and flow cytometry- based, assay platforms.
Densmore, Douglas, Lashkaripour, Ali, Ortiz, Luis   +2 more
core  

Cyclic nucleotide specificity of the activator and catalytic sites of a cGMP-stimulated cGMP phosphodiesterase from Dictyostelium discoideum [PDF]

, 1985
The cellular slime mold Dictyostelium discoideum has an intracellular phosphodiesterase which specifically hydrolyzes cGMP. The enzyme is activated by low cGMP concentrations, and is involved in the reduction of chemoattractant-mediated elevations of ...
Baraniak, Janina,, Bulgakov, Roman,, Haastert, Peter J.M. van,, Jastorff, Bernd,, Kesbeke, Fanja,, Morr, Michael,, Petridis, Georg,, Seela, Frank,, Stec, Wojciech J.,   +8 more
core   +2 more sources

A Human Homolog of Angiotensin-converting Enzyme

Journal of Biological Chemistry, 2000
A novel human zinc metalloprotease that has considerable homology to human angiotensin-converting enzyme (ACE) (40% identity and 61% similarity) has been identified.
S. Tipnis, N. Hooper, R. Hyde, E. Karran, G. Christie, A. Turner   +5 more
semanticscholar   +1 more source

The Insulin-Degrading Enzyme from Structure to Allosteric Modulation: New Perspectives for Drug Design [PDF]

, 2023
Grazia Raffaella Tundo, Giuseppe Grasso, Marco Persico, О. В. Ткачук, Francesco Bellia, Alessio Bocedi, Stefano Marini, Mariacristina Parravano, Grazia Graziani, Caterina Fattorusso, Diego Sbardella   +10 more
openalex   +1 more source

Linked dimers of the AAA+ ATPase Msp1 reveal energetic demands and mechanistic plasticity for substrate extraction from lipid bilayers

FEBS Letters, EarlyView.
Cells must clear mislocalized or faulty proteins from membranes to survive. The AAA+ ATPase Msp1 performs this task, but dissecting how its six subunits work together is challenging. We engineered linked dimers with varied numbers of functional subunits to reveal how Msp1 subunits cooperate and use energy to extract proteins from the lipid bilayer ...
Deepika Gaur, Brian Acquaviva, Baylee A. Smith, Nathan Walker, Isabella Walter, Matthew L. Wohlever   +5 more
wiley   +1 more source

Covalently bound substrate at the regulatory site triggers allosteric enzyme activation [PDF]

, 2008
The mechanism by which the enzyme pyruvate decarboxylase from yeast is activated allosterically has been elucidated. A total of seven three-dimensional structures of the enzyme, of enzyme variants or of enzyme complexes form two yeast species (three of ...
Georg Wille, Manfred Weiss, Michael Spinka, Ralph Golbik, Steffen Kutter, Stephan Kö   +5 more
core   +1 more source

Machine Learning in Enzyme Engineering

ACS Catalysis, 2019
Enzyme engineering plays a central role in developing efficient biocatalysts for biotechnology, biomedicine, and life sciences.
S. Mazurenko, Z. Prokop, J. Damborský
semanticscholar   +1 more source

Reciprocal control of viral infection and phosphoinositide dynamics

FEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source