Results 101 to 110 of about 6,423,741 (359)
The pH-static enzyme sensor : An ISFET-based enzyme sensor, insensitive to the buffer capacity of the sample [PDF]
, 1987 An ISFET-based urea sensor is combined with a noble-metal electrode which provides continuous coulometric titration of the products of the enzymatic reaction.
Bergveld, Piet, Schoot, Bart H. van der
core +3 more sources
, 2018 In recent years, enzyme immobilization has been presented as a powerful tool for the improvement of enzyme properties such as stability and reusability. However, the type of support material used plays a crucial role in the immobilization process due to ...
J. Zdarta +3 more
semanticscholar +1 more source
Calpain small subunit homodimerization is robust and calcium‐independent
FEBS Letters, EarlyView.Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy +4 more
wiley +1 more source
Cellulase Production by Wild-type Aspergillus niger, Penicillium chrysogenum and Trichoderma harzianum Using Waste Cellulosic Materials [PDF]
, 2005 Waste cellulosic materials (corncob, sawdust and sugarcane pulp) and crystalline cellulose induced cellulase production in wild strains of Aspergillus niger, Penicillium chrysogenum and Trichoderma harzianum isolated from a wood-waste dump in Lagos ...
Chinedu, S. N., Okochi, V. I.
core
Machine learning applied to enzyme turnover numbers reveals protein structural correlates and improves metabolic models. [PDF]
, 2018 Knowing the catalytic turnover numbers of enzymes is essential for understanding the growth rate, proteome composition, and physiology of organisms, but experimental data on enzyme turnover numbers is sparse and noisy.
Desouki, Abdelmoneim Amer +9 more
core +4 more sources
Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
FEBS Letters, EarlyView.A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
wiley +1 more source
Microbial Cell Factories, 2019 Background The methylotrophic yeast Pichia pastoris is well-known for the production of a broad spectrum of functional types of heterologous proteins including enzymes, antigens, engineered antibody fragments, and next gen protein scaffolds and many ...
Xueyun Zheng +6 more
doaj +1 more source
Microbial Cell Factories, 2020 Chitinase and chitin-oligosaccaride can be used in multiple field, so it is important to develop a high-yield chitinase producing strain. Here, a recombinant Pichia pastoris with 4 copies of ChiA gene from Bacillus licheniformis and co-expression of ...
Wen Song +5 more
doaj +1 more source
A Human Homolog of Angiotensin-converting Enzyme
Journal of Biological Chemistry, 2000 A novel human zinc metalloprotease that has considerable homology to human angiotensin-converting enzyme (ACE) (40% identity and 61% similarity) has been identified.
S. Tipnis +5 more
semanticscholar +1 more source
Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system
FEBS Letters, EarlyView.Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley +1 more source