Results 121 to 130 of about 6,423,741 (359)

Rapid generation of recombinant Pseudomonas putida secondary metabolite producers using yTREX

Synthetic and Systems Biotechnology, 2017
Microbial secondary metabolites represent a rich source of valuable compounds with a variety of applications in medicine or agriculture. Effective exploitation of this wealth of chemicals requires the functional expression of the respective biosynthetic ...
Andreas Domröse, Robin Weihmann, Stephan Thies, Karl-Erich Jaeger, Thomas Drepper, Anita Loeschcke   +5 more
doaj   +1 more source

Absolute Metabolite Concentrations and Implied Enzyme Active Site Occupancy in Escherichia coli

Nature Chemical Biology, 2009
Absolute metabolite concentrations are critical to a quantitative understanding of cellular metabolism, as concentrations impact both the free energies and rates of metabolic reactions.
Bryson D. Bennett, Elizabeth H Kimball, Melissa Gao, R. Osterhout, S. Van Dien, Joshua D. Rabinowitz   +5 more
semanticscholar   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Linking neurogenesis, oligodendrogenesis, and myelination defects to neurodevelopmental disruption in primary mitochondrial disorders

FEBS Letters, EarlyView.
Mitochondrial remodeling shapes neural and glial lineage progression by matching metabolic supply with demand. Elevated OXPHOS supports differentiation and myelin formation, while myelin compaction lowers mitochondrial dependence, revealing mitochondria as key drivers of developmental energy adaptation.
Sahitya Ranjan Biswas, Porter L. Tomsick, Alicia M. Pickrell, Paul D. Morton   +3 more
wiley   +1 more source

Liver enzyme alteration: a guide for clinicians

Canadian Medical Association Journal, 2005
ISOLATED ALTERATIONS OF BIOCHEMICAL MARKERS OF LIVER DAMAGE in a seemingly healthy patient can present a challenge for the clinician. In this review we provide a guide to interpreting alterations to liver enzyme levels.
E. Giannini, R. Testa, V. Savarino
semanticscholar   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

A pH Dependant Switch in DHP Oxidation Mechanism [PDF]

, 2017
Dehaloperoxidase (DHP) is a multifunctional enzyme found in Amphitrite ornata, a sediment-dwelling marine worm. This enzyme possess the structure of a traditional hemoglobin enzyme and serves as the primary oxygen carrier in A.
Fehr, Travis
core   +1 more source

Design of minibinder proteins as universal antagonists against canine and human TNFα

Communications Biology
In this study, we resolve the complex structure of canine TNFα (cTNFα) trimer bound to nanobody molecules at a resolution of 3.1 Å using cryo-electron microscopy.
Jun Weng, Zhiyong Wu, Banbin Xing, Yang Hu, Xiaoyu Hu, Meng Mei, Jiaxin Xu, Mengqing Lu, Yibin Chen, Lin Wei, Ke Ming, Zhizheng Wang, Zhuang Li, Zigong Wei   +13 more
doaj   +1 more source

The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes

FEBS Letters, EarlyView.
Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga, Toshiki Takahashi, Akiko Kuma, Hiroyuki Kawahara   +3 more
wiley   +1 more source

A Whole-Process Visible Strategy for the Preparation of Rhizomucor miehei Lipase with Escherichia coli Secretion Expression System and the Immobilization

Microbial Cell Factories
Background Rhizomucor miehei (RM) lipase is a regioselective lipase widely used in food, pharmaceutical and biofuel industries. However, the high cost and low purity of the commercial RM lipase limit its industrial applications.
Mingjun Yang, Xianhui Su, Jun Yang, Zhiwen Lu, Jie Zhou, Fei Wang, Yang Liu, Lixin Ma, Chao Zhai   +8 more
doaj   +1 more source