Results 51 to 60 of about 3,915,254 (313)

The [2Fe‐2S] cluster of mitochondrial outer membrane protein mitoNEET has an O2‐regulated nitric oxide access tunnel

open access: yesFEBS Letters, EarlyView.
The mitochondrial outer membrane iron–sulphur ([Fe‐S]) protein mitoNEET is a target of the type‐2 diabetes drug pioglitazone. Its unknown molecular function is linked to respiratory complex activity and mitochondrial function. We discovered that O2 protects the mitoNEET [2Fe‐2S] cluster against NO oxidation and desensitization towards reduction by H2S.
Thao Nghi Hoang   +9 more
wiley   +1 more source

Absolute diffusion measurements of active enzyme solutions by NMR [PDF]

open access: yes, 2018
The diffusion of enzymes is of fundamental importance for many biochemical processes. Enhanced or directed enzyme diffusion can alter the accessibility of substrates and the organization of enzymes within cells. Several studies based on fluorescence correlation spectroscopy (FCS) report enhanced diffusion of enzymes upon interaction with their ...
arxiv   +1 more source

Information propagation in time through allosteric signaling [PDF]

open access: yesPhys. Rev. Research 2, 023367 (2020), 2019
Naively, one expects the information communicated by an enzyme downstream within a signaling network, in which the enzyme is embedded, to grow monotonically with the enzyme's rate of product formation. However, here we observe that this does not necessarily hold true for allosterically regulated enzymes, often observed in signaling networks.
arxiv   +1 more source

Characterization of fungal carbonyl sulfide hydrolase belonging to clade D β‐carbonic anhydrase

open access: yesFEBS Letters, EarlyView.
Here, we performed a functional analysis of the fungal enzymes belonging to clade D of the β‐class carbonic anhydrase family (β‐D‐CA). The β‐D‐CAs in the basidiomycete Gloeophyllum trabeum and the ascomycete Trichoderma harzianum showed very low activity in the hydration of CO2 but exhibited high activity in the hydrolysis of carbonyl sulfide (COS ...
Ryuka Iizuka   +6 more
wiley   +1 more source

Multiple strategies to improve the yield of chitinase a from Bacillus licheniformis in Pichia pastoris to obtain plant growth enhancer and GlcNAc

open access: yesMicrobial Cell Factories, 2020
Chitinase and chitin-oligosaccaride can be used in multiple field, so it is important to develop a high-yield chitinase producing strain. Here, a recombinant Pichia pastoris with 4 copies of ChiA gene from Bacillus licheniformis and co-expression of ...
Wen Song   +5 more
doaj   +1 more source

Cholecystitis & An Enzyme Study [PDF]

open access: yes, 2011
Cholecystitis is inflammation of the gallbladder that develops in short time usually when gallstone obstructs the cystic duct. Patients over the passage of time land to chronic cholecystitis.
Anil Batta, Int J Cur Biomed Phar Res.
core  

Comparison of Cholesterol Lowering Diets: Apple, Casein Cytochrom P450 protein and Cholesterol 7α Hydroxylase Activities in Hamsters [PDF]

open access: yes, 2010
Lithogenic diet, casein and apple fiber diets were fed to hamsters for 3-5 weeks. For control group, animals were fed on normal Purina chow without any supplement.
Rakesh Sharma, Rakesh K. Tandon
core   +2 more sources

A histidine‐rich extension of the mitochondrial F0 subunit ATP6 from the ice worm Mesenchytraeus solifugus increases ATP synthase activity in bacteria

open access: yesFEBS Letters, EarlyView.
The glacier ice worm Mesenchytraeus solifugus survives year‐round at 0 °C. Its ATP6 subunit, which forms a regulatory component of the proton pore in mitochondrial ATP synthase, has a carboxy‐terminal extension not found in any other organism examined to date. Here, we show that fusion of this extension to the homologous AtpB protein in E. coli results
Truman Dunkley   +2 more
wiley   +1 more source

Purification and properties of a HeLa cell enzyme able to remove the 5'- terminal protein from poliovirus RNA [PDF]

open access: yes, 1980
Using a rapid phenol extraction assay, an enzyme was purified from uninfected HeLa cells that can cleave the 5'-terminal protein (VPg) from poliovirus RNA. Both cytoplasmic and nuclear extracts had enzymes with similar behavior.
Ambros, Victor, Baltimore, David
core  

Parallel versus off-pathway Michaelis-Menten mechanism for single-enzyme kinetics of a fluctuating enzyme

open access: yes, 2015
Recent fluorescence spectroscopy measurements of the turnover time distribution of single-enzyme turnover kinetics of $\beta$-galactosidase provide evidence of Michaelis-Menten kinetics at low substrate concentration.
Dua, Arti   +2 more
core   +2 more sources

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