Results 91 to 100 of about 6,536,634 (394)

From omics to AI—mapping the pathogenic pathways in type 2 diabetes

FEBS Letters, EarlyView.
Integrating multi‐omics data with AI‐based modelling (unsupervised and supervised machine learning) identify optimal patient clusters, informing AI‐driven accurate risk stratification. Digital twins simulate individual trajectories in real time, guiding precision medicine by matching patients to targeted therapies.
Siobhán O'Sullivan, Lu Qi, Pierre Zalloua   +2 more
wiley   +1 more source

Inputs and outputs of poly(ADP-ribosyl)ation: Relevance to oxidative stress

Redox Biology, 2014
Oxidative stress can cause DNA breaks which induce activation of the DNA nick sensor enzyme poly(ADP-ribose) polymerase-1 (PARP-1), part of the 17 member PARP enzyme family.
Csaba Hegedűs, László Virág
doaj   +1 more source

KDAC8 with High Basal Velocity Is Not Activated by N-Acetylthioureas. [PDF]

PLoS ONE, 2016
Lysine deacetylases (KDACs) are enzymes that reverse the post-translational modification of lysine acetylation. Recently, a series of N-acetylthioureas were synthesized and reported to enhance the activity of KDAC8 with a fluorogenic substrate.
Tasha B Toro, Subramanya Pingali, Thao P Nguyen, Destane S Garrett, Kyra A Dodson, Kyara A Nichols, Rashad A Haynes, Florastina Payton-Stewart, Terry J Watt   +8 more
doaj   +1 more source

Activation of thiamin diphosphate in enzymes [PDF]

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1998
Activation of the coenzyme ThDP was studied by measuring the kinetics of deprotonation at the C2 carbon of thiamin diphosphate in the enzymes pyruvate decarboxylase, transketolase, pyruvate dehydrogenase complex, pyruvate oxidase, in site-specific mutant enzymes and in enzyme complexes containing coenzyme analogues by proton/deuterium exchange detected
Hübner, Gerhard, Tittmann, Kai, Killenberg-Jabs, Margrit, Schäffner, Jörg, Spinka, Michael, Neef, Holger, Kern, Dorothee, Kern, Gunther, Schneider, Gunter, Wikner, Christer, Ghisla, Sandro   +10 more
openaire   +4 more sources

The anabolic steroid stanozolol is a potent inhibitor of human MutT homolog 1

FEBS Letters, EarlyView.
MutT homolog 1 (MTH1) is a member of the NUDIX superfamily of enzymes and is an anticancer drug target. We show that stanozolol (Stz), an anabolic steroid, is an unexpected nanomolar inhibitor of MTH1. The X‐ray crystal structure of the human MTH1–Stz complex reveals a unique binding scaffold that could be utilized for future inhibitor development ...
Emma Scaletti Hutchinson, Robert Gustafsson Westergren, Ingrid Almlöf, Ann‐Sofie Jemth, Martin Scobie, Ulrika Warpman Berglund, Thomas Helleday, Pål Stenmark   +7 more
wiley   +1 more source

Thermostable neutral metalloprotease from Geobacillus sp. EA1 does not share thermolysin's preference for substrates with leucine at the P1′ position

FEBS Letters, EarlyView.
Knowing how proteases recognise preferred substrates facilitates matching proteases to applications. The S1′ pocket of protease EA1 directs cleavage to the N‐terminal side of hydrophobic residues, particularly leucine. The S1′ pocket of thermolysin differs from EA's at only one position (leucine in place of phenylalanine), which decreases cleavage ...
Grant R. Broomfield, Torsten Kleffmann, David J. Saul, Sigurd M. Wilbanks   +3 more
wiley   +1 more source

Expression and regulation of cyclic nucleotide phosphodiesterases in human and rat pancreatic islets. [PDF]

PLoS ONE, 2010
As shown by transgenic mouse models and by using phosphodiesterase 3 (PDE3) inhibitors, PDE3B has an important role in the regulation of insulin secretion in pancreatic β-cells. However, very little is known about the regulation of the enzyme.
Emilia Heimann, Helena A Jones, Svante Resjö, Vincent C Manganiello, Lena Stenson, Eva Degerman   +5 more
doaj   +1 more source

Effects of detergents on activity, thermostability and aggregation of immobilized lipases [PDF]

, 2014
Predmetom tejto diplomovej práce bolo štúdium vplyvu detergentov na aktivitu, termostabilitu a agregáciu voľnej a imobilizovanej formy komerčného preparátu lipázy izolovanej z mikroskopickej huby Rhizopus arrhizus. Teoretická časť obsahuje ucelenú rešerš
Bančáková, Anna
core  

Discovery of Escherichia coli methionyl-tRNA synthetase mutants for efficient labeling of proteins with azidonorleucine in vivo [PDF]

, 2009
Incorporation of noncanonical amino acids into cellular proteins often requires engineering new aminoacyl-tRNA synthetase activity into the cell. A screening strategy that relies on cell-surface display of reactive amino acid side-chains was used to ...
Goddard, William A., III, Mechulam, Yves, Schmitt, Emmanuelle, Tanrikulu, I. Caglar, Tirrell, David A.   +4 more
core   +5 more sources

Crystal Structures of Salmonella typhimurium Biodegradative Threonine Deaminase and Its Complex with CMP Provide Structural Insights into Ligand-induced Oligomerization and Enzyme Activation*

Journal of Biological Chemistry, 2006
Two different pyridoxal 5′-phosphate-containing l-threonine deaminases (EC 4.3.1.19), biosynthetic and biodegradative, which catalyze the deamination of l-threonine to α-ketobutyrate, are present in Escherichia coli and Salmonella typhimurium ...
D. Simanshu, H. Savithri, M. Murthy
semanticscholar   +1 more source