Results 201 to 210 of about 13,881,946 (357)

Resveratrol Sustains Insulin-Degrading Enzyme Activity toward Aβ42

ACS Omega, 2018
Claire A. Krasinski, Valerie A. Ivancic, Qiuchen Zheng, Donald E. Spratt, Noel D. Lazo   +4 more
doaj   +1 more source

Regulating Enzyme Activity via Microaggregates Mediated by Phase Separation. [PDF]

Adv Sci (Weinh)
Wang Y, Yuan J, Dai N, Ji Y, Yang W, Li X, Yan S, Yan J.   +7 more
europepmc   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Mycorrhizal Inoculation Enhances Drought Tolerance in Potato (Solanum tuberosum L.) by Modulating Antioxidant Enzyme Activity and Related Gene Expression. [PDF]

Biology (Basel)
Ettlili S, Aroca R, Djebali N, Labidi S, Jeddi FB.   +4 more
europepmc   +1 more source

Autophagosome marker, LC3, is released extracellularly via several distinct pathways

FEBS Open Bio, EarlyView.
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito, Masashi Arakawa, Koki Maeda, Eiji Morita   +3 more
wiley   +1 more source

Phosphate ions modulate enzyme activity and epistatic effects in two clavulanic acid-resistant β-lactamase mutants. [PDF]

Protein Sci
Radojković M, Koene SF, Chikunova A, Florea BI, Natarajan SV, Boyle AL, Ubbink M.   +6 more
europepmc   +1 more source

Hands‐on protocol for preparing water‐soluble fractions from agri‐food samples for NMR‐based metabolomics analysis

FEBS Open Bio, EarlyView.
This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso, Jaime Hiniesta‐Valero, Alejandra Guerra‐Castellano, Miguel A. De la Rosa, Irene Díaz‐Moreno   +4 more
wiley   +1 more source

The Effects of Drugs on CYP3A Enzyme Activity and Protein Expression Through Ubiquitination Modification Regulation. [PDF]

Basic Clin Pharmacol Toxicol
Wang F, Gu X, Hong H, Xia Q, Zhang Y, Song Y, Li B, Fan P.   +7 more
europepmc   +1 more source

SIRT4 positively regulates autophagy via ULK1, but independently of HDAC6 and OPA1

FEBS Open Bio, EarlyView.
Cells expressing SIRT4 (H161Y), a catalytically inactive mutant of the sirtuin SIRT4, fail to upregulate LC3B‐II and exhibit a reduced autophagic flux under stress conditions. Interestingly, SIRT4(H161Y) promotes phosphorylation of ULK1 at S638 and S758 that are associated with inhibition of autophagy initiation.
Isabell Lehmkuhl, Khawar Amin, Lydia Gabriel, Nils Hampel, Afshin Iram, Julia Hesse, Constanze Wiek, Jasmin Thuy Vy Nguyen, Helmut Hanenberg, Jürgen Scheller, M. Reza Ahmadian, Björn Stork, Doreen M. Floss, Roland P. Piekorz   +13 more
wiley   +1 more source

Single-Molecule DNA Tweezers Enable Programmable Control of Enzyme Activity via Arbitrary Molecular Cues. [PDF]

Angew Chem Int Ed Engl
Godhulayyagari S, Nixon SR, Samanta D.
europepmc   +1 more source