Results 91 to 100 of about 5,306,282 (389)

The thioredoxin‐like and one glutaredoxin domain are required to rescue the iron‐starvation phenotype of HeLa GLRX3 knock out cells

FEBS Letters, EarlyView.
Glutaredoxin (Grx) 3 proteins contain a thioredoxin domain and one to three class II Grx domains. These proteins play a crucial role in iron homeostasis in eukaryotic cells. In human Grx3, at least one of the two Grx domains, together with the thioredoxin domain, is essential for its function in iron metabolism.
Laura Magdalena Jordt, Manuela Gellert, Finja Zelms, Sander Bekeschus, Christopher Horst Lillig   +4 more
wiley   +1 more source

Cellulase immobilization on superparamagnetic nanoparticles for reuse in cellulosic biomass conversion [PDF]

, 2016
Current cellulosic biomass hydrolysis is based on the one-time use of cellulases. Cellulases immobilized on magnetic nanocarriers offer the advantages of magnetic separation and repeated use for continuous hydrolysis. Most immobilization methods focus on
Fernando Segato, Mark Wilkins, null null, Qing Song, Rolf Prade, Yu Mao   +5 more
core   +3 more sources

In vivo evidence for glycyl radical insertion into a catalytically inactive variant of pyruvate formate‐lyase

FEBS Letters, EarlyView.
Dimeric pyruvate formate‐lyase cleaves pyruvate using a radical‐based mechanism. G734 serves as a radical storage location, and the radical is transferred to the catalytic C419 residue. Mutation of the C418‐C419 pair causes loss of enzyme activity, but does not impede radical introduction onto G734. Therefore, cis‐ but not trans‐radical transfer occurs
Michelle Kammel, A. F. Volker Wagner, R. Gary Sawers   +2 more
wiley   +1 more source

Disruption of SETD3‐mediated histidine‐73 methylation by the BWCFF‐associated β‐actin G74S mutation

FEBS Letters, EarlyView.
The β‐actin G74S mutation causes altered interaction of actin with SETD3, reducing histidine‐73 methylation efficiency and forming two distinct actin variants. The variable ratio of these variants across cell types and developmental stages contributes to tissue‐specific phenotypical changes. This imbalance may impair actin dynamics and mechanosensitive
Anja Marquardt, Marcus S. Münchhoff, Jacqueline Krohn, Philip M. Palarz, Manuel H. Taft, Johannes N. Greve, Nataliya Di Donato, Falk F. R. Buettner, Dietmar J. Manstein   +8 more
wiley   +1 more source

Molecular and biochemical characterization of carbonic anhydrases of Paracoccidioides

Genetics and Molecular Biology
Carbonic anhydrases (CA) belong to the family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide to bicarbonate. In the present work, we characterized the cDNAs of four Paracoccidioides CAs (CA1, CA2, CA3, and CA4).
Mariana Vieira Tomazett, Fabiana Fonseca Zanoelo, Elisa Flávia Cardoso Bailão, Alexandre Melo Bailão, Clayton Luiz Borges, Célia Maria de Almeida Soares   +5 more
doaj   +1 more source

Assays for Investigating deSUMOylation Enzymes [PDF]

Current Protocols in Molecular Biology, 2012
AbstractPost‐translational modifications by the SUMO (Small Ubiquitin‐like MOdifier) family of proteins are recently discovered essential regulatory mechanisms. All SUMO proteins are synthesized as larger precursors that are matured by SUMO‐specific proteases, known as SENPs, which remove several C‐terminal amino acids of SUMO to expose the Gly‐Gly ...
Ikenna G. Madu, Yuan Chen
openaire   +3 more sources

Circulating histones as clinical biomarkers in critically ill conditions

FEBS Letters, EarlyView.
Circulating histones are emerging as promising biomarkers in critical illness due to their diagnostic, prognostic, and therapeutic potential. Detection methods such as ELISA and mass spectrometry provide reliable approaches for quantifying histone levels in plasma samples.
José Luis García‐Gimenez, Juan Carlos Ruiz‐Rodríguez, Ricard Ferrer, Raquel Durá, Antonio Artigas, Iván Bajaña, David Bolado López de Andujar, Irene Cánovas‐Cervera, Adrián Ceccato, Luis Chiscano‐Camón, Elena Climent‐Martinez, Georgia García Fernández, Gemma Goma, Verónica Monforte, Beatriz Quevedo‐Sánchez, Adolf Ruiz‐Sanmartín, Antonio Sierra‐Rivera, Nieves Carbonell Monleón   +17 more
wiley   +1 more source

A rare PHKA2 variant (p.G991A) identified in a patient with ketotic hypoglycemia

JIMD Reports, 2019
We describe the case of a 4‐year‐old boy who suffered from frequent ketotic hypoglycemia (KH) but did not have hepatomegaly or elevated liver enzyme levels. However, the patient was found to have a rare variant in the PHKA2 gene. To detect the underlying
Yasuhiko Ago, Hideo Sugie, Tokiko Fukuda, Hiroki Otsuka, Hideo Sasai, Mina Nakama, Elsayed Abdelkreem, Toshiyuki Fukao   +7 more
doaj   +1 more source

Using Enzyme-Linked Aptamer Assay to Detect Hepatitis B Surface Antigen in Comparison to Enzyme-Linked Immunosorbent Assay

مجله دانشکده پزشکی اصفهان, 2018
Background: Clinical diagnostic kits for detecting hepatitis B are based on antibodies, and have inefficient sensitivity, stability, and cost. Therefore, researches about aptamers and using them instead of antibodies may make these defects up.
Hamidreza Mianesaz, Hossein Khanahmad, Mina Mirian, Maryam Boshtam, Seyed Nezamoddin Hoseini   +4 more
doaj   +1 more source

Enzyme-Linked Immunosorbent-Assay for Deoxynivalenol (DON)

Toxins, 2011
Deoxynivalenol (DON), one of the trichothecene mycotoxins, is a worldwide contaminant of wheat and barley, especially when infected by Fusarium graminearum, the causative agent of an epidemic wheat disease called Fusarium Head Blight. Because of the high
Jianrong Shi, Jianhong Xu, Fang Ji, Hua Li   +3 more
doaj   +1 more source