Results 11 to 20 of about 1,624,448 (258)
Bio-Protocol, 2013 LDH (Lactate dehydrogenase) enzyme catalyzes the reversible conversion of pyruvate to lactate using NAD+ as a cofactor. Although the physiological significance of lactate accumulation in tumor cells, a dead-end product in cellular metabolism, is ...
Di Zhao +3 more
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Neuronal ceroid lipofuscinoses in children
Annals of Indian Academy of Neurology, 2021 Background: The neuronal ceroid lipofuscinoses (NCL) constitute a group of gray matter neurodegenerative disorders characterized by the accumulation of ceroid lipopigment in lysosomes in neurons and other cell types.
Mahesh Kamate +3 more
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Enzyme-linked immunosorbent assay for urinary albumin at low concentrations [PDF]
, 1987 We describe an enzyme-linked immunosorbent assay (ELISA) for urinary albumin. It requires only commercially available reagents, can detect as little as 16 micrograms of albumin per liter, and analytical recovery ranges from 92 to 116%.
Ress, K. M. +4 more
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Bio-Protocol, 2014 Reductive dehalogenation has been found primarily in anaerobic communities and is originally thought to rarely occur in aerobes. A reductive dehalogenase (BhbA) was characterized from an aerobic strain of Comamonas sp.
Kai Chen, Jiandong Jiang
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Separations, 2022 The search for new anti-inflammatory drugs with less side effects requires simple, fast and reliable screening methods. In this context, we have developed a sensitive thin-layer chromatographic (TLC) assay on silica gel plates to detect cyclooxygenase-2 (
Aurélie Urbain +2 more
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Biochemical and Biophysical Characterisation of the Hepatitis E Virus Guanine-7-Methyltransferase
Molecules, 2022 Hepatitis E virus (HEV) is an understudied pathogen that causes infection through fecal contaminated drinking water and is prominently found in South Asian countries.
Preeti Hooda +13 more
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Brazilian Archives of Biology and Technology, 2013 A gene encoding NAD+-dependent sorbitol dehydrogenase (SDH) in peach fruit was cloned and expressed in Escherichia coli. Recombinant SDH protein with 6×His-tagged was localized exclusively in the cytoplasmic soluble fraction of E.
Zhen Sun +3 more
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A method for assaying deubiquitinating enzymes [PDF]
Biological Procedures Online, 1998 A general method for the assay of deubiquitinating enzymes was described in detail using (125)I-labeled ubiquitin-fused alphaNH-MHISPPEPESEEEEEHYC (referred to as Ub-PESTc) as a substrate. Since the tyrosine residue in the PESTc portion of the fusion protein was almost exclusively radioiodinated under a mild labeling condition, such as using IODO-BEADS,
Lee Jae Il +6 more
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Molecules, 2022 Aphid, Aphis craccivora Koch (Hemiptera: Aphididae), is a major sap-sucking insect pest of leguminous crops and also transmits plant viruses, leading to economic yield loss.
Shudh Kirti Dolma +2 more
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Biochemical and computational study of an alginate lyase produced by Pseudomonas aeruginosa strain S21 [PDF]
Iranian Journal of Basic Medical Sciences, 2020 Objective(s): Alginates play a key role in mucoid Pseudomonas aeruginosa colonization, biofilm formation, and driving out of cationic antibiotics. P. aeruginosa alginate lyase (AlgL) is a periplasmic enzyme that is necessary for alginate synthesis and ...
Firoozeh Piroozmand +3 more
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