Validation of an Indirect Immunofluorescence Assay and Commercial Q Fever Enzyme-Linked Immunosorbent Assay for Use in Macropods [PDF]
, 2022 Kangaroos are considered to be an important reservoir of Q fever in Australia, although there is limited knowledge on the true prevalence and distribution of coxiellosis in Australian macropod populations.
Tolpinrud, A +38 more
core +1 more source
Simultaneous isolation of mRNA and native protein from minute samples of cells
BioTechniques, 2014 Precious biological samples often lack a sufficient number of cells for multiple procedures, such as extraction of mRNA while maintaining protein in a non-denatured state suitable for subsequent characterization.
Tonny Studsgaard Petersen +1 more
doaj +1 more source
Enzymatic Analysis of Yeast Cell Wall-Resident GAPDH and Its Secretion
mSphere, 2020 In yeast, many proteins are found in both the cytoplasmic and extracellular compartments, and consequently it can be difficult to distinguish nonconventional secretion from cellular leakage.
Michael J. Cohen +2 more
doaj +1 more source
The Development of Assays for Heparanase Enzymatic Activity: Towards a Gold Standard
Molecules, 2018 The enzyme heparanase, an endo-β-glucuronidase, degrades heparan sulfate (HS) chains on the cell surface and in the extracellular matrix. Heparanase regulates numerous biological processes that drive tumour growth, metastasis and angiogenesis.
Mohit Chhabra, Vito Ferro
doaj +1 more source
Lateral-Flow Assay for Rapid Serodiagnosis of Human Leptospirosis [PDF]
, 2001 An assay device for the rapid detection of Leptospira-specific immunoglobulin M (IgM) antibodies in human sera is presented. The sensitivity (85.8%) and specificity (93.6%) of the assay compared well (91.9% agreement) with those of an IgM enzyme-linked ...
Abdoel, Theresia H +10 more
core +1 more source
Biochemistry and Molecular Biology Education, 2004 AbstractTerms to be familiar with before you start to solve the test: signal transduction, growth factor signalling, nerve growth factor (NGF), epidermal growth factor (EGF), mitogen‐activated protein kinases (MAPKs), extracellular signal‐regulated kinases (ERKs), MAPK/ERK kinase (MEK), SDS‐PAGE, [γ‐32P]ATP, autoradiography, caspases, protein kinases ...
openaire +2 more sources
A membrane-based ELISA assay for the herbicide Isoproturon in soil samples [PDF]
, 2012 A membrane based enzyme linked immunosorbent assay (MELISA) for the detection of a common herbicide, isoproturon is described. A heterogeneous competitive ELISA was the format chosen for isoproturon detection.
Baskeyfield, Damian E. H. +7 more
core +1 more source
Schematic diagram of enzyme-linked immunosorbent assay.
, 2023 Schematic diagram of enzyme-linked immunosorbent assay.
Naoya Kawanoue (13129390) +8 more
core +1 more source
Development of a Universal NADH Detection Assay for High Throughput Enzyme Evolution Using Fluorescence Activated Droplet Sorting [PDF]
, 2022 Directed evolution is an enzyme engineering approach based on the generation and screening of large mutagenesis libraries, with a view to discovering enzymes with improved properties such as activity, specificity or stability.
Gerassimos, Kolaitis +10 more
core +2 more sources
A rare PHKA2 variant (p.G991A) identified in a patient with ketotic hypoglycemia
JIMD Reports, 2019 We describe the case of a 4‐year‐old boy who suffered from frequent ketotic hypoglycemia (KH) but did not have hepatomegaly or elevated liver enzyme levels. However, the patient was found to have a rare variant in the PHKA2 gene. To detect the underlying
Yasuhiko Ago +7 more
doaj +1 more source