Results 31 to 40 of about 1,624,448 (258)
Conductimetric Enzyme Assays [PDF]
A direct reading, six channel conductivity recording apparatus has been constructed and used to assay enzymes, including some of those found in muscle. This paper gives the essential theory of the method, and indicates that it is a highly versatile assay system. It may be applicable whenever ionic substrates are involved, but this is more easily tested
openaire +2 more sources
Enzyme immunoassay (EIA)/enzyme-linked immunosorbent assay (ELISA [PDF]
This brief note addresses the historical background of the invention of the enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA).
Rudolf M. Lequin, Rudolf M Lequin
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Catalase is a key antioxidative enzyme, and a deficiency or malfunction of catalase is hypothesized to be related to various diseases. To investigate catalase activity, it is important to use reliable methods and experimental protocols enabling ...
Michal Szczepanczyk +3 more
doaj +1 more source
The Kulka resorcinol assay (Kulka, R.G., Biochemistry 1956, 63, 542⁻548) for ketoses has been widely used in the literature but suffers from two major disadvantages: (a) it employs large amounts of potentially harmful reagents for a general biology
Shreya Shaw, Robin Ghosh
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Capillary electrophoresis using laser-induced fluorescence detection (CE-LIF) is one of the most sensitive separation tools among electrical separation methods.
Binh Thanh Nguyen, Min-Jung Kang
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Serotonin (5-HT) is a hormone and neurotransmitter that modulates neural activity as well as a wide range of other physiological processes including cardiovascular function, bowel motility, and platelet aggregation.
Nibal Betari +14 more
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Aflatoxin determination in peanut butter by enzyme-linked immunosorbent assay
High specificity, high titre antisera to aflatoxins B1 and G1 have been produced by immunisation of rabbits with a bovine serum albumin-aflatoxin B1 conjugate. The antiserum has been used to set up an indirect, double antibody microtitration plate enzyme-
Kang, A.S., Chan, H.W.S., Morgan, M.R.A.
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Enzyme Linked Immunosorbent Assay (ELISA) Technique Guideline [PDF]
ELISA (Enzyme-linked immunosorbent assay) is a technique used to assess the quantification of peptide, protein, antibody and hormone levels, based on the principle of antigen-antibody binding.
Patricia Wulandari +3 more
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Cloning and Overexpression of Strictosidine β-D-Glucosidase Gene Short Sequence from Catharanthus roseus in Escherichia coli [PDF]
Purpose: Strictosidine-β-D-glucosidase (SGD) is considered as a key enzyme in the production of bisindole alkaloids in Catharanthus roseus. The present study illustrated the production of a short sequence of this enzyme in Escherichia coli without codon ...
Ahmed Saeed Arafa +4 more
doaj +1 more source
Oxidative Power: Tools for Assessing LPMO Activity on Cellulose
Lytic polysaccharide monooxygenases (LPMOs) have sparked a lot of research regarding their fascinating mode-of-action. Particularly, their boosting effect on top of the well-known cellulolytic enzymes in lignocellulosic hydrolysis makes them industrially
Federica Calderaro +2 more
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