Results 31 to 40 of about 1,624,448 (258)

Conductimetric Enzyme Assays [PDF]

open access: yesEuropean Journal of Biochemistry, 1971
A direct reading, six channel conductivity recording apparatus has been constructed and used to assay enzymes, including some of those found in muscle. This paper gives the essential theory of the method, and indicates that it is a highly versatile assay system. It may be applicable whenever ionic substrates are involved, but this is more easily tested
openaire   +2 more sources

Enzyme immunoassay (EIA)/enzyme-linked immunosorbent assay (ELISA [PDF]

open access: yes, 2005
This brief note addresses the historical background of the invention of the enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA).
Rudolf M. Lequin, Rudolf M Lequin
core   +1 more source

Comparison of Oxygen Electrode Chronoamperometry and Spectrophotometry for Determination of Catalase Activity

open access: yesOxygen, 2023
Catalase is a key antioxidative enzyme, and a deficiency or malfunction of catalase is hypothesized to be related to various diseases. To investigate catalase activity, it is important to use reliable methods and experimental protocols enabling ...
Michal Szczepanczyk   +3 more
doaj   +1 more source

A Modified Kulka Micromethod for the Rapid and Safe Analysis of Fructose and 1-Deoxy-d-xylulose-5-phosphate

open access: yesMetabolites, 2018
The Kulka resorcinol assay (Kulka, R.G., Biochemistry 1956, 63, 542⁻548) for ketoses has been widely used in the literature but suffers from two major disadvantages: (a) it employs large amounts of potentially harmful reagents for a general biology
Shreya Shaw, Robin Ghosh
doaj   +1 more source

Application of Capillary Electrophoresis with Laser-Induced Fluorescence to Immunoassays and Enzyme Assays

open access: yesMolecules, 2019
Capillary electrophoresis using laser-induced fluorescence detection (CE-LIF) is one of the most sensitive separation tools among electrical separation methods.
Binh Thanh Nguyen, Min-Jung Kang
doaj   +1 more source

Inhibition of Tryptophan Hydroxylases and Monoamine Oxidase-A by the Proton Pump Inhibitor, Omeprazole—In Vitro and In Vivo Investigations

open access: yesFrontiers in Pharmacology, 2020
Serotonin (5-HT) is a hormone and neurotransmitter that modulates neural activity as well as a wide range of other physiological processes including cardiovascular function, bowel motility, and platelet aggregation.
Nibal Betari   +14 more
doaj   +1 more source

Aflatoxin determination in peanut butter by enzyme-linked immunosorbent assay

open access: yes, 1986
High specificity, high titre antisera to aflatoxins B1 and G1 have been produced by immunisation of rabbits with a bovine serum albumin-aflatoxin B1 conjugate. The antiserum has been used to set up an indirect, double antibody microtitration plate enzyme-
Kang, A.S., Chan, H.W.S., Morgan, M.R.A.
core   +1 more source

Enzyme Linked Immunosorbent Assay (ELISA) Technique Guideline [PDF]

open access: yes, 2021
ELISA (Enzyme-linked immunosorbent assay) is a technique used to assess the quantification of peptide, protein, antibody and hormone levels, based on the principle of antigen-antibody binding.
Patricia Wulandari   +3 more
core   +1 more source

Cloning and Overexpression of Strictosidine β-D-Glucosidase Gene Short Sequence from Catharanthus roseus in Escherichia coli [PDF]

open access: yesAdvanced Pharmaceutical Bulletin, 2019
Purpose: Strictosidine-β-D-glucosidase (SGD) is considered as a key enzyme in the production of bisindole alkaloids in Catharanthus roseus. The present study illustrated the production of a short sequence of this enzyme in Escherichia coli without codon ...
Ahmed Saeed Arafa   +4 more
doaj   +1 more source

Oxidative Power: Tools for Assessing LPMO Activity on Cellulose

open access: yesBiomolecules, 2021
Lytic polysaccharide monooxygenases (LPMOs) have sparked a lot of research regarding their fascinating mode-of-action. Particularly, their boosting effect on top of the well-known cellulolytic enzymes in lignocellulosic hydrolysis makes them industrially
Federica Calderaro   +2 more
doaj   +1 more source

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