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Micro- and nanochamber array system for single enzyme assays [PDF]

open access: yesScientific Reports, 2023
Arrays of small reaction containers, ranging from 624 femtoliters (10–15 L) to 270 attoliters (10–18 L), for capturing a single enzyme molecule and measuring the activity were developed along with a new reversible sealing system based on a pneumatic ...
Kazuki Iijima   +3 more
doaj   +2 more sources

Synthesis of Phenylboronic Acid-Functionalized Magnetic Nanoparticles for Sensitive Soil Enzyme Assays [PDF]

open access: yesMolecules, 2022
Soil enzymes, such as invertase, urease, acidic phosphatase and catalase, play critical roles in soil biochemical reactions and are involved in soil fertility.
Can Li   +7 more
doaj   +2 more sources

Time-Dependent Kinetic Complexities in Enzyme Assays: A Review [PDF]

open access: yesBiomolecules
In the present review, the importance of analyzing full progress curves in enzyme assays is discussed. The atypical kinetic behavior that can be potentially displayed by enzymes in the performance of an activity assay, as well as the models explaining ...
Juan Luis Rendón, Juan Pablo Pardo
doaj   +2 more sources

Application of Capillary Electrophoresis with Laser-Induced Fluorescence to Immunoassays and Enzyme Assays [PDF]

open access: yesMolecules, 2019
Capillary electrophoresis using laser-induced fluorescence detection (CE-LIF) is one of the most sensitive separation tools among electrical separation methods.
Binh Thanh Nguyen, Min-Jung Kang
doaj   +2 more sources

Easily Fabricated Microfluidic Devices Using Permanent Marker Inks for Enzyme Assays [PDF]

open access: yesMicromachines, 2016
In this communication, we describe microfluidic paper analytical devices (μPADs) easily fabricated from commercially available Sharpie ink permanent markers on chromatography paper to colorimetrically detect glucose using glucose oxidase (GOx).
Coreen Gallibu   +3 more
doaj   +2 more sources

Rational Design for Enhanced Acyltransferase Activity in Water Catalyzed by the Pyrobaculum calidifontis VA1 Esterase

open access: yesMicroorganisms, 2021
Biocatalytic transesterification is commonly carried out employing lipases in anhydrous organic solvents since hydrolases usually prefer hydrolysis over acyl transfer in bulk water.
Amanda Staudt   +7 more
doaj   +1 more source

New gold pincer-type complexes induce caspase-dependent apoptosis in human cancer cells in vitro [PDF]

open access: yesVojnosanitetski Pregled, 2021
Background/Aim. The use of cisplatin as a chemotherapeutic opened the door to the new metal-based drug research. New complexes containing metals such as platinum, palladium, ruthenium and gold have recently been analyzed as potential antitumor agents ...
Zarić Milan M.   +9 more
doaj   +1 more source

Using Steady-State Kinetics to Quantitate Substrate Selectivity and Specificity: A Case Study with Two Human Transaminases

open access: yesMolecules, 2022
We examined the ability of two human cytosolic transaminases, aspartate aminotransferase (GOT1) and alanine aminotransferase (GPT), to transform their preferred substrates whilst discriminating against similar metabolites.
Alessio Peracchi, Eugenia Polverini
doaj   +1 more source

Monitoring Protein Denaturation of Egg White Using Passive Microwave Radiometry (MWR)

open access: yesDiagnostics, 2022
Passive microwave radiometry (MWR) is a measurement technique based on the detection of passive radiation in the microwave spectrum of different objects.
Igor Goryanin   +3 more
doaj   +1 more source

Enzymatic Methods for Salivary Biomarkers Detection: Overview and Current Challenges

open access: yesMolecules, 2021
Early detection is a key factor in patient fate. Currently, multiple biomolecules have been recognized as biomarkers. Nevertheless, their identification is only the starting line on the way to their implementation in disease diagnosis.
Alonso Ornelas-González   +3 more
doaj   +1 more source

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