Application of Capillary Electrophoresis with Laser-Induced Fluorescence to Immunoassays and Enzyme Assays [PDF]
Molecules, 2019 Capillary electrophoresis using laser-induced fluorescence detection (CE-LIF) is one of the most sensitive separation tools among electrical separation methods.
Binh Thanh Nguyen, Min-Jung Kang
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Enzyme assays with supramolecular chemosensors - the label-free approach. [PDF]
RSC Adv, 2022 Enzyme activity measurements are essential for many research areas, e.g., for the identification of inhibitors in drug discovery, in bioengineering of enzyme mutants for biotechnological applications, or in bioanalytical chemistry as parts of biosensors.
Nilam M, Hennig A.
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Label-Free Fluorescent Kinase and Phosphatase Enzyme Assays with Supramolecular Host-Dye Pairs. [PDF]
ChemistryOpen, 2019 The combination of the macrocyclic hosts p‐sulfonatocalix[4]arene and cucurbit[7]uril with the fluorescent dyes lucigenin and berberine affords two label‐free enzyme assays for the detection of kinase and phosphatase activity by fluorescence monitoring ...
Liu YC +4 more
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Micro- and nanochamber array system for single enzyme assays [PDF]
Scientific Reports, 2023 Arrays of small reaction containers, ranging from 624 femtoliters (10–15 L) to 270 attoliters (10–18 L), for capturing a single enzyme molecule and measuring the activity were developed along with a new reversible sealing system based on a pneumatic ...
Kazuki Iijima +3 more
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Synthesis of Phenylboronic Acid-Functionalized Magnetic Nanoparticles for Sensitive Soil Enzyme Assays [PDF]
Molecules, 2022 Soil enzymes, such as invertase, urease, acidic phosphatase and catalase, play critical roles in soil biochemical reactions and are involved in soil fertility.
Can Li +7 more
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Plant Methods, 2021 Background Enzyme assays have widespread applications in drug discovery from plants to natural products. The appropriate use of blanks in enzyme assays is important for assay baseline-correction, and the correction of false signals associated with ...
Chintha Lankatillake +6 more
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Enzyme assays for synthesis and degradation of 2-5As and other 2'-5' oligonucleotides. [PDF]
BMC Biochem, 2015 BackgroundThe 5′-triphosphorylated, 2′-5′-linked oligoadenylate polyribonucleotides (2-5As) are central to the interferon-induced antiviral 2-5A system.
Poulsen JB +3 more
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Time-Dependent Kinetic Complexities in Enzyme Assays: A Review [PDF]
BiomoleculesIn the present review, the importance of analyzing full progress curves in enzyme assays is discussed. The atypical kinetic behavior that can be potentially displayed by enzymes in the performance of an activity assay, as well as the models explaining ...
Juan Luis Rendón, Juan Pablo Pardo
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Easily Fabricated Microfluidic Devices Using Permanent Marker Inks for Enzyme Assays [PDF]
Micromachines, 2016 In this communication, we describe microfluidic paper analytical devices (μPADs) easily fabricated from commercially available Sharpie ink permanent markers on chromatography paper to colorimetrically detect glucose using glucose oxidase (GOx).
Coreen Gallibu +3 more
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Kinetic modelling: an integrated approach to analyze enzyme activity assays
Plant Methods, 2017 Background In general, enzyme activity is estimated from spectrophotometric data, by taking the slope of the linear part of the progress curve describing the rate of change in the substrate or product monitored.
Jelena Boeckx +3 more
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