Results 21 to 30 of about 4,824,034 (339)

Production of Extracellular Protease from Bacterial Co-cultures using Solid State Fermentation

open access: yesBioscientific Review, 2020
Proteases (also known as peptidases or proteinases) are hydrolytic enzymes that cleave proteins into amino acids. They comprise 60% of the total industrial usage of enzymes worldwide and can be obtained from many sources.
Nabiha Naeem Sheikhs   +2 more
doaj   +1 more source

Expression of matrix metalloproteinases (MMPs) and their inhibitor (TIMP) genes on mRNA and protein levels in oral squamous cell carcinoma

open access: yesNowotwory, 2021
INTRODUCTION: To investigate the mRNA and protein expression of MMP-2, MMP-9, MMP-7 and their tissue inhibitor TIMP-2 in tissue specimens with oral squamous cell cancer (OSCC) and in healthy tissues.
Artur Wróbel-Roztropiński   +5 more
doaj   +1 more source

Evaluation of Nucleocapsid and Spike Protein-Based Enzyme-Linked Immunosorbent Assays for Detecting Antibodies against SARS-CoV-2

open access: yesJournal of Clinical Microbiology, 2020
At present, PCR-based nucleic acid detection cannot meet the demands for coronavirus infectious disease (COVID-19) diagnosis. Two hundred fourteen confirmed COVID-19 patients who were hospitalized in the General Hospital of Central Theater Command of the
Wanbing Liu   +11 more
semanticscholar   +1 more source

Amino Acid Racemase Enzyme Assays

open access: yesBio-Protocol, 2014
Amino acid racemases are enzymes that invert the α-carbon stereochemistry of amino acids (AAs), interconverting amino acids between their L- and D-enantiomers in a reversible reaction. In bacteria, they are known to have catabolic physiological functions
Atanas Radkov, Luke Moe
doaj   +1 more source

Undiagnosed Hepatitis B and C Virus Infection at a Teaching Hospital in Rawalpindi

open access: yesJournal of Pure and Applied Microbiology, 2020
Uncontrolled prevalence of hepatitis B and C virus infections is known to be a global health problem. Many cases are undiagnosed yet. So, this study focusses on the undiagnosed existence of viral infection of hepatitis B (HBV) and Hepatitis C infection
Muhammad Imran Khan   +8 more
doaj   +1 more source

Structure activity relationship studies on Amb639752: toward the identification of a common pharmacophoric structure for DGKα inhibitors

open access: yesJournal of Enzyme Inhibition and Medicinal Chemistry, 2020
A series of analogues of Amb639752, a novel diacylglycerol kinase (DGK) inhibitor recently discovered by us via virtual screening, have been tested. The compounds were evaluated as DGK inhibitors on α, θ, and ζ isoforms, and as antagonists on serotonin ...
Suresh Velnati   +11 more
doaj   +1 more source

Interaction of Cucurbit[7]uril With Protease Substrates: Application to Nanosecond Time-Resolved Fluorescence Assays

open access: yesFrontiers in Chemistry, 2020
We report the use of the macrocyclic host cucurbit[7]uril (CB7) as a supramolecular additive in nanosecond time-resolved fluorescence (Nano-TRF) assays for proteases to enhance the discrimination of substrates and products and, thereby, the sensitivity ...
Andreas Hennig   +3 more
doaj   +1 more source

In vivo and in vitro protein digestibility of formulated feeds for Artemesia longinaris (Crustacea, Penaeidae)

open access: yesBrazilian Archives of Biology and Technology, 2009
This study was undertaken to determine the in vivo crude protein apparent digestibility in the prawn Artemesia longinaris, using feeds with 0.25% of chromic oxide and animal (fish meal, meat and bone meal and squid protein concentrate) and plant (soybean
Analía Verónica Fernández Gimenez   +3 more
doaj   +1 more source

Conductimetric Enzyme Assays [PDF]

open access: yesEuropean Journal of Biochemistry, 1971
A direct reading, six channel conductivity recording apparatus has been constructed and used to assay enzymes, including some of those found in muscle. This paper gives the essential theory of the method, and indicates that it is a highly versatile assay system. It may be applicable whenever ionic substrates are involved, but this is more easily tested
openaire   +2 more sources

POLYPHENOL OXIDASE AND PEROXIDASE ENZYME ASSAYS IN SWEET POTATO CULTIVARS HARVESTED AT DIFFERENT TIMES

open access: yesRevista Caatinga, 2019
Enzyme assays are based on methodologies described in the literature. However, the enzyme kinetics must be adjusted to obtain more reliable results.
Samara Lopes de Almeida   +5 more
semanticscholar   +1 more source

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