Energy-starved microbes in deep marine sediments subsist at near-zero growth for thousands of years, yet the mechanisms for their subsistence are unknown because no model strains have been cultivated from most of these groups.
Jordan T. Bird +11 more
doaj +1 more source
Specificity and Kinetics of Haloalkane Dehalogenase [PDF]
Haloalkane dehalogenase converts halogenated alkanes to their corresponding alcohols. The active site is buried inside the protein and lined with hydrophobic residues. The reaction proceeds via a covalent substrate-enzyme complex.
Janssen, Dick B., +2 more
core +5 more sources
Current State of the Art of Newborn Screening for Lysosomal Storage Disorders
Prospective full-population newborn screening for multiple lysosomal storage disorders (LSDs) is currently practiced in a few NBS programs, and several others are actively pursuing this course of action.
David S. Millington, Deeksha S. Bali
doaj +1 more source
Optimisation of the enzyme-linked lectin assay for enhanced glycoprotein and glycoconjugate analysis [PDF]
Lectin’s are proteins capable of recognising and binding to specific oligosaccharide tructures found on glycoproteins and other biomoloecules. As such they have found tility for glycoanalytical applications.
Afrough +48 more
core +2 more sources
Optimizing pH for Soil Enzyme Assays Reveals Important Biochemical Functions in Low pH Soil
Soil enzyme assays are often used as indicators of potential biological functions. The objective of this study was to understand enzyme activity across a range of soil pH.
Tandra D. Fraser +4 more
semanticscholar +1 more source
The Urea Carboxylase and Allophanate Hydrolase Activities of Urea Amidolyase Are Functionally Independent [PDF]
Urea amidolyase (UAL) is a multifunctional biotin-dependent enzyme that contributes to both bacterial and fungal pathogenicity by catalyzing the ATP-dependent cleavage of urea into ammonia and CO2.
Bahler +48 more
core +2 more sources
Identifying Novel Inhibitors of RpFabG in Typhus-inducing Rickettsia prowazekii [PDF]
Epidemic typhus is a rickettsial disease that is contracted via ticks and lice found on the flying squirrel. The disease is caused by Rickettsia prowazekii, an intracellular, gram-negative coccobacillus.
Panatpur, Aparna
core +1 more source
Biochemical Markers and Enzyme Assays for Herbicide Mode of Action and Resistance Studies
Herbicides inhibit biochemical and physiological processes or both with lethal consequences. The target sites of these small molecules are usually enzymes involved in primary metabolic pathways or proteins carrying out essential physiological functions ...
F. Dayan +6 more
semanticscholar +1 more source
Genome-wide analysis of the UDP-glucose dehydrogenase gene family in Arabidopsis, a key enzyme for matrix polysaccharides in cell walls [PDF]
Arabidopsis cell walls contain large amounts of pectins and hemicelluloses, which are predominantly synthesized via the common precursor UDP-glucuronic acid.
Klinghammer, Michaela, Tenhaken, Raimund
core +1 more source
Enzyme Immunoassay and Enzyme-Linked Immunosorbent Assay [PDF]
inTrOducTiOn Enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA) are both widely used as diagnostic tools in medicine and as quality control measures in various industries; they are also used as analytical tools in biomedical research for the detection and quantification of specific antigens or antibodies in a given sample. These two
Gan, Stephanie D., Patel, Kruti R.
openaire +2 more sources

