Results 201 to 210 of about 5,705,534 (301)

Efficient synthesis of l-DOPA in <i>Escherichia coli</i> via cofactor and enzyme engineering. [PDF]

Synth Syst Biotechnol
Deng L, Ping J, Liu Z, Linghu K, Zhang H, Shan X, Zeng W, Li J, Zhou J.   +8 more
europepmc   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Enzyme engineering

, 1998
Dordick, Jonathan S., Laskin, Allen I., International Enzyme Engineering Conference   +2 more
core  

Advances in microbial biofuel production by metabolic and enzyme engineering, synthetic biology, metagenomics, and genome editing applications. [PDF]

Emerg Top Life Sci
Alam SS, Mehdi A, Zafar A, Ali S, Rehman AU, Liaqat I, Peng L, Kanwal F, Afzal S, Haq IU, Aftab MN.   +10 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Accelerated enzyme engineering by machine-learning guided cell-free expression. [PDF]

Nat Commun
Landwehr GM, Bogart JW, Magalhaes C, Hammarlund EG, Karim AS, Jewett MC.   +5 more
europepmc   +1 more source

De novo synthesis of short‐chain aldehydes and hydrocarbons secreted by the brown marmorated stink bug Halyomorpha halys

FEBS Open Bio, EarlyView.
The brown marmorated stink bug, Halyomorpha halys, mainly secretes 4‐oxo‐(E)‐2‐hexenal, (E)‐2‐decenal, dodecane and tridecane, a mixture representative of that released by many heteropterans. We demonstrated that H. halys can synthesise these secretory components de novo using dietary glucose.
Haruna Fujimori, Koji Noge
wiley   +1 more source

Combination of enzyme engineering and quorum sensing system for efficient de novo biosynthesis of β-arbutin in <i>Escherichia coli</i>. [PDF]

Synth Syst Biotechnol
Liu Z, Liu D, Deng L, LingHu K, Shan X, Zhou J, Zeng W.   +6 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Growth-coupled continuous directed evolution by MutaT7 enables efficient and automated enzyme engineering. [PDF]

Appl Environ Microbiol
Deng Y, Etheridge K, Ran X, Maurais HE, Sarpeshkar R.   +4 more
europepmc   +1 more source