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Engineering enzyme specificity
Current Opinion in Chemical Biology, 1998Protein engineering is the application of knowledge to design and alter protein function and structure. Although powerful methods, from specific to random, have been developed for the redesign of protein architecture, their successful application is dependent on the information known about the protein.
J L, Harris, C S, Craik
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Applied Biochemistry and Biotechnology, 1983
Enzyme research and development efforts have been shaped by the tools and concepts available for enzyme production and utilization. A new phase of enzymology characterized by the production of modified protein catalysts has begun, made possible by recombinant DNA technology.
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Enzyme research and development efforts have been shaped by the tools and concepts available for enzyme production and utilization. A new phase of enzymology characterized by the production of modified protein catalysts has begun, made possible by recombinant DNA technology.
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Engineering enzymes for stability
Current Opinion in Structural Biology, 1996There have been many recent developments in elaborating the approaches for stabilizing enzymes by stabilizing the folding state, destabilizing the unfolded state and altering the kinetics of unfolding. However, these represent a series of rules of thumb rather than the reliable principles that would be expected of 'engineering'.
A, Shaw, R, Bott
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Engineered metalloregulation in enzymes
Trends in Biochemical Sciences, 1992Protein engineering of metal-dependent enzyme activity is now possible due to the wealth of information available about metalloproteins. The results emerging from these studies provide insight into our understanding of the chemistry of metals in macromolecular environments as well as the biology of metal-protein interactions.
J N, Higaki, R J, Fletterick, C S, Craik
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Enzyme Catalysis and Engineering
2004Lignocellulosic biomass is a valuable and plentiful feedstock commodity and its high cellulose and hemicellulose content (about 80% of total) provides considerable potential for inexpensive sugars production. However, enzymatic deconstruction of these polysaccharides remains a costly prospect.
Mike, Himmel, David, Wilson
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Engineering Cytochrome P450 Enzymes
Chemical Research in Toxicology, 2007The last 20 years have seen the widespread and routine application of methods in molecular biology such as molecular cloning, recombinant protein expression, and the polymerase chain reaction. This has had implications not only for the study of toxicological mechanisms but also for the exploitation of enzymes involved in xenobiotic clearance.
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Engineering Enzymes for Biocatalysis
Recent Patents on Biotechnology, 2007Protein engineering techniques have been available for over two decades beginning with the development of methods for genetic engineering. Since that time, the engineering of enzymes has advanced rapidly along with a revolution in the range and efficiency of new techniques and strategies for designing and evolving proteins in the laboratory.
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Engineering Therapeutic Enzymes
2017Biologics constitute a rapidly growing category of pharmaceutical drug products. With over 100 clinically approved therapeutics and many more in development, protein-based compounds represent an important subset among these biomacromolecular drug candidates, ranging from antibodies, anticoagulants, growth factors, hormones, interferons, and ...
Stefan Lutz +2 more
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