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Development of enzyme technology and enzyme engineering in China
Applied Biochemistry and Biotechnology, 1982Enzymes have been utilized in China for thousands of years for the production of various foods and alcoholic beverages. Today China manufactures and uses enzymes for not only the traditional areas of application, but is expanding the use of enzymes for a variety of nonfood areas.
Z, Shuzheng, X, Jiali
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Rational engineering of enzyme stability
Journal of Biotechnology, 2004During the past 15 years there has been a continuous flow of reports describing proteins stabilized by the introduction of mutations. These reports span a period from pioneering rational design work on small enzymes such as T4 lysozyme and barnase to protein design, and directed evolution.
Eijsink, V.G. +7 more
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Enzyme Engineering by Targeted Libraries
2013This review outlines the strategies we apply for directed enzyme evolution using targeted libraries, namely, libraries that diversify specific residues with predefined mutational compositions. The theoretical grounds underlining the design of such libraries are described, including the mutational load, the ratio of beneficial versus deleterious ...
Moshe, Goldsmith, Dan S, Tawfik
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Enzyme Engineering: Current Trends and Future Perspectives
Food Reviews International, 2021Baljinder Kaur, Tawseef Ahmad
exaly
Transketolase enzyme discovery and engineering
Transketolases (TKs) are important C-C bond forming enzymes that in vivo transfer a two carbon ketol unit to the acceptors d-ribose-5-phosphate or d-erythrose-4-phosphate. There is significant interest in biocatalytic applications where frequently the donor β-hydroxypyruvic acid is used.Alessia, Tonoli +3 more
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