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Development of enzyme technology and enzyme engineering in China
Applied Biochemistry and Biotechnology, 1982Enzymes have been utilized in China for thousands of years for the production of various foods and alcoholic beverages. Today China manufactures and uses enzymes for not only the traditional areas of application, but is expanding the use of enzymes for a variety of nonfood areas.
Z, Shuzheng, X, Jiali
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Rational engineering of enzyme stability
Journal of Biotechnology, 2004During the past 15 years there has been a continuous flow of reports describing proteins stabilized by the introduction of mutations. These reports span a period from pioneering rational design work on small enzymes such as T4 lysozyme and barnase to protein design, and directed evolution.
Eijsink, V.G. +7 more
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Evolving strategies for enzyme engineering
Current Opinion in Structural Biology, 2005Directed evolution is a common technique to engineer enzymes for a diverse set of applications. Structural information and an understanding of how proteins respond to mutation and recombination are being used to develop improved directed evolution strategies by increasing the probability that mutant sequences have the desired properties.
Bloom, Jesse D. +5 more
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Enzyme engineering: accomplishments and prospects
Endeavour, 1981Abstract Developments in enzyme technology could lead to a completely new type of chemical industry utilizing moderate conditions of pH, temperature, and pressure to produce, from wastes or by-products, high yields of complex chemicals without generating new waste products that pollute the environment. The new tools of this branch are immobilized, as
D, Thomas, G, Gellf
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Enzyme Engineering by Targeted Libraries
2013This review outlines the strategies we apply for directed enzyme evolution using targeted libraries, namely, libraries that diversify specific residues with predefined mutational compositions. The theoretical grounds underlining the design of such libraries are described, including the mutational load, the ratio of beneficial versus deleterious ...
Moshe, Goldsmith, Dan S, Tawfik
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Enzyme Engineering: Current Trends and Future Perspectives
Food Reviews International, 2021Baljinder Kaur, , Tawseef Ahmad
exaly