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Applied Biochemistry and Biotechnology, 1983
Enzyme research and development efforts have been shaped by the tools and concepts available for enzyme production and utilization. A new phase of enzymology characterized by the production of modified protein catalysts has begun, made possible by recombinant DNA technology.
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Enzyme research and development efforts have been shaped by the tools and concepts available for enzyme production and utilization. A new phase of enzymology characterized by the production of modified protein catalysts has begun, made possible by recombinant DNA technology.
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Engineering enzyme specificity
Current Opinion in Chemical Biology, 1998Protein engineering is the application of knowledge to design and alter protein function and structure. Although powerful methods, from specific to random, have been developed for the redesign of protein architecture, their successful application is dependent on the information known about the protein.
J L, Harris, C S, Craik
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SUBTILISIN: AN ENZYME DESIGNED TO BE ENGINEERED
Trends in Biochemical Sciences, 1988Abstract Almost every property of subtilisin, a simple bacterial serine protease, has been altered by protein engineering including its catalysis, substrate specificity, pH/rate profile, and stability to oxidative, thermal and alkaline inactivation.
J A, Wells, D A, Estell
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Enzyme Catalysis and Engineering
2004Lignocellulosic biomass is a valuable and plentiful feedstock commodity and its high cellulose and hemicellulose content (about 80% of total) provides considerable potential for inexpensive sugars production. However, enzymatic deconstruction of these polysaccharides remains a costly prospect.
Mike, Himmel, David, Wilson
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Engineering Enzymes for Biocatalysis
Recent Patents on Biotechnology, 2007Protein engineering techniques have been available for over two decades beginning with the development of methods for genetic engineering. Since that time, the engineering of enzymes has advanced rapidly along with a revolution in the range and efficiency of new techniques and strategies for designing and evolving proteins in the laboratory.
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Protein engineering the surface of enzymes
Journal of Biotechnology, 1998The protein surface is the interface through which a protein senses the external world. Its composition of charged, polar and hydrophobic residues is crucial for the stability and activity of the protein. The charge state of seven of the twenty naturally occurring amino acids is pH dependent. A total of 95% of all titratable residues are located on the
Petersen, Steffen B. +7 more
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Engineering Cytochrome P450 Enzymes
Chemical Research in Toxicology, 2007The last 20 years have seen the widespread and routine application of methods in molecular biology such as molecular cloning, recombinant protein expression, and the polymerase chain reaction. This has had implications not only for the study of toxicological mechanisms but also for the exploitation of enzymes involved in xenobiotic clearance.
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Development of enzyme technology and enzyme engineering in China
Applied Biochemistry and Biotechnology, 1982Enzymes have been utilized in China for thousands of years for the production of various foods and alcoholic beverages. Today China manufactures and uses enzymes for not only the traditional areas of application, but is expanding the use of enzymes for a variety of nonfood areas.
Z, Shuzheng, X, Jiali
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Rational engineering of enzyme stability
Journal of Biotechnology, 2004During the past 15 years there has been a continuous flow of reports describing proteins stabilized by the introduction of mutations. These reports span a period from pioneering rational design work on small enzymes such as T4 lysozyme and barnase to protein design, and directed evolution.
Eijsink, V.G. +7 more
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Evolving strategies for enzyme engineering
Current Opinion in Structural Biology, 2005Directed evolution is a common technique to engineer enzymes for a diverse set of applications. Structural information and an understanding of how proteins respond to mutation and recombination are being used to develop improved directed evolution strategies by increasing the probability that mutant sequences have the desired properties.
Bloom, Jesse D. +5 more
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