Results 41 to 50 of about 285,607 (309)

Phosphoinositides and inositol phosphates as molecular glues

FEBS Letters, EarlyView.
Inositol phosphates (IPs) and phosphoinositides (PIPs) regulate diverse eukaryotic processes. Beyond recruiting signaling proteins or acting as structural cofactors, recent studies suggest they mediate protein–protein interactions as natural molecular glues.
Aleshia Seaton‐Terry, Alexander J. Cutright, Mong Na Claire Loi, Jessica Martin, Joseph W. Gilligan, Jakub N. Kubina, Michael J. Arambula, Susanna Tanada Palmu, Daniella Zhou, Raymond D. Blind   +9 more
wiley   +1 more source

IMPDH inhibition enhances cytarabine efficacy in SAMHD1‐expressing leukaemia cells via guanine nucleotide depletion

Molecular Oncology, EarlyView.
Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla, Christopher Dirks, Caroline Eiden, Sonja K. Fesenmayer, Femke M. Hormann, Yolande Klootsema, Ingrid Lilienthal, Si Min Zhang, Nikolas Herold, Sean G. Rudd   +9 more
wiley   +1 more source

Structure of the N-terminal gyrase B fragment in complex with ADP⋅Pi reveals rigid-body motion induced by ATP hydrolysis [PDF]

, 2014
Type II DNA topoisomerases are essential enzymes that catalyze topological rearrangement of double-stranded DNA using the free energy generated by ATP hydrolysis.
Stanger, Frédéric V., Frédéric V Stanger, Tilman Schirmer, Dehio, Christoph, Schirmer, Tilman, Christoph Dehio, Frédéric V. Stanger (585551), Frédéric V. Stanger, Tilman Schirmer (585552), Christoph Dehio (200951)   +9 more
core   +1 more source

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

Enzymatic hydrolysis of potato starch and ethanol production [PDF]

Hemijska Industrija, 2004
The hydrolysis of potato starch using one (Termamyl or Fungamyl) and two combined (Termamyl and Supersan) commercial enzyme preparations and ethanol production from the hydrolysates obtained using the yeast Saccharomyces cerevisiae were studied.
Lazić Miodrag L., Rašković Suzana, Stanković Mihajlo Z., Veljković Vlada B.   +3 more
doaj   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

Process control of rice protein enzymolysis by field monitoring

Current Research in Biotechnology
By devising a novel near-infrared (NIR) in-situ online monitoring system, this study proposed a new process control method for rice protein (RP) enzymatic hydrolysis in order to address the challenge of monitoring certain chemical indices in real-time ...
Yanhua Ding, Peng Han, Haile Ma, Janet Quaisie, Jamila A. Tuly   +4 more
doaj   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Catalytic mechanism of alpha-phosphate attack in dUTPase is revealed by X-ray crystallographic snapshots of distinct intermediates, 31P-NMR spectroscopy and reaction path modelling.

, 2013
Enzymatic synthesis and hydrolysis of nucleoside phosphate compounds play a key role in various biological pathways, like signal transduction, DNA synthesis and metabolism.
Bodor, A., Vértessy, Beáta G, Kele, Z., Wilmanns, Matthias, Perczel, András, Perczel, A., Vértessy, Beáta G., Rosta, Edina, Zagyva, Imre, Grolmusz, Vince I., Barabás, Orsolya, Németh, Veronika, Vertessy, B. G., Grolmusz, Vince I, Barabas, O., Grolmusz, V. I., Vértessy G., Beáta, Kele, Zoltán, Szabadka, Zoltán, Zagyva, I., Wilmanns, M., Szabadka, Z., Rosta, E., Nemeth, V., Bodor, Andrea   +24 more
core   +1 more source

Aiming for the complete utilization of sugar-beet pulp: Examination of the effects of mild acid and hydrothermal pretreatment followed by enzymatic digestion [PDF]

, 2011
Background Biomass use for the production of bioethanol or platform chemicals requires efficient breakdown of biomass to fermentable monosaccharides. Lignocellulosic feedstocks often require physicochemical pretreatment before enzymatic hydrolysis can ...
Kühnel Stefan, Kuhnel, S., Schols, H.A., Schols Henk A, Stefan Kühnel, Harry Gruppen, Henk A Schols, Gruppen Harry, Gruppen, H.   +8 more
core   +1 more source