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Enzyme immobilization on heparin
Journal of Biomedical Materials Research, 1978AbstractWe describe the preparation and some of the properties of heparin‐bound α‐chymotrypsin that were obtained via activation of heparin with water‐soluble carbodiimide. Immobilized enzyme has unchanged kinetic characteristics toward low‐molecular‐weight and macromolecular substrates.
V P, Torchilin +4 more
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Enzyme immobilization in porous solid supports—penetration of immobilized enzyme
Biotechnology and Bioengineering, 1984AbstractThe process of enzyme immobilization under the diffusion‐controlled regime (i.e., fast attachment of enzyme compared to its diffusion) is modeled and theoretically solved in this article. Simple and compact solutions for the penetration depth of immobilized enzyme and the bulk enzyme concentration versus time are presented.
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Pellicular immobilized enzymes
Biochimica et Biophysica Acta (BBA) - Enzymology, 1974Abstract Methods for the preparation of shell-structured immobilized enzymes supported by a fluid impervious spherical core are outlined. The preparation of such trypsin resins by coreticulation of the enzyme with a copolymer of maleic anhydride and vinyl methylester in situ on the surface of glass beads is described and some properties of the ...
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Nanoporous Gold for Enzyme Immobilization
2010Nanoporous gold (NPG) is a material of emerging interest for immobilization of biomolecules, especially enzymes. The material provides a high surface area form of gold that is suitable for physisorption or for covalent modification by self-assembled monolayers.
Keith J, Stine +2 more
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Enzyme Immobilization by Adsorption
2003Much has already been written on the subject of biocatalyst (cell/enzyme) immobilization, and it is not the function of a text such as this to go into all the various aspects of the field. For general reviews on the area, the reader is referred to refs. 1-3.
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Enzyme Immobilization by Entrapment
2003Entrapment methods of immobilization are mostly used in cell immobilization procedures, but have found some application with enzymes, particularly when the enzyme is essentially a dead cell or crude homogenate(1). In theory the entrapped enzyme is not attached to the polymer; its free diffusion is merely restrained. In practice, however, some or all of
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Kinetic Measurements for Enzyme Immobilization
2010Enzyme kinetics is the study of the chemical reactions that are catalyzed by enzymes, with a focus on their reaction rates. The study of an enzyme's kinetics considers the various stages of activity, reveals the catalytic mechanism of the enzyme, correlates its value to assay conditions, and describes how a drug or a poison might inhibit the enzyme ...
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Enzyme co-immobilization: Always the biocatalyst designers' choice…or not?
Biotechnology Advances, 2021Sara Arana-Peña +2 more
exaly