Results 71 to 80 of about 2,214,190 (390)

A new approach to evaluate immobilization of β-galactosidase on Eupergit® C: structural, kinetic, and thermal characterization

open access: yesQuímica Nova, 2014
This study aimed to evaluate β-galactosidase immobilization. For this purpose, the ionic strength of the buffer, reaction time, amount of the immobilization support, and pH were evaluated by a central composite design. Assay 8, which consisted of 1.5 mol
Anna R. C. Braga   +4 more
doaj   +1 more source

Pengaruh Ph, Waktu dan Konsentrasi dalam Matriks Kitosan Mg Terhadap Immobilisasi Enzim Papain [PDF]

open access: yes, 2018
Shrimp shell waste can be processed into chitosan. Immobilization of enzymes is carried out to facilitate the separation of the enzyme and the resulting product.
Ayu Lestari Warsito, Sri   +1 more
core  

Unlocking the potential of tumor‐derived DNA in urine for cancer detection: methodological challenges and opportunities

open access: yesMolecular Oncology, EarlyView.
Urine is a rich source of biomarkers for cancer detection. Tumor‐derived material is released into the bloodstream and transported to the urine. Urine can easily be collected from individuals, allowing non‐invasive cancer detection. This review discusses the rationale behind urine‐based cancer detection and its potential for cancer diagnostics ...
Birgit M. M. Wever   +1 more
wiley   +1 more source

Process Optimization and Application of Lipase Immobilized on Phenol Amino Modified MCM-41

open access: yesShipin gongye ke-ji
In order to construct a new catalytic system of immobilized lipase and improve the stability of enzyme, the phenol amino modified ordered mesoporous material (MCM-41) was introduced for the study of lipase immobilization.
Qian LI   +8 more
doaj   +1 more source

An enzyme‐linked immunosorbent assay (ELISA)‐based activity assay for AMP‐activated protein kinase (AMPK)

open access: yesFEBS Open Bio, EarlyView.
Measuring AMP‐activated protein kinase (AMPK) activity in vitro is crucial for testing AMPK activators or inhibitors with therapeutic potential. Here, we report an enzyme‐linked immunosorbent assay (ELISA)‐based AMPK activity assay with simple steps and high sensitivity, which offers a simple, robust, and cost‐effective alternative to traditional ...
Trezze P. Nguyen, Shangze Lyu, Yang Liu
wiley   +1 more source

Regulating Protein Immobilization During Cell‐Free Protein Synthesis in Hyaluronan Microgels

open access: yesAdvanced Biology, EarlyView.
Bifunctional microgels carrying a linear DNA template and Ni2+‐activated NTA moieties are used as platform for cell‐free protein synthesis and in situ protein immobilization. By varying the concentration of NTA moieties in the microgels, the amount of GFP‐His immobilized inside the microgel and released to the microgel environment can be regulated ...
Anika Kaufmann   +2 more
wiley   +1 more source

Effects of detergents on activity, thermostability and aggregation of immobilized lipases [PDF]

open access: yes, 2014
Predmetom tejto diplomovej práce bolo štúdium vplyvu detergentov na aktivitu, termostabilitu a agregáciu voľnej a imobilizovanej formy komerčného preparátu lipázy izolovanej z mikroskopickej huby Rhizopus arrhizus. Teoretická časť obsahuje ucelenú rešerš
Bančáková, Anna
core  

Effect of peppermint oil on serum lipid peroxidation and hepatic enzymes after immobility stress in mice [PDF]

open access: yes, 2012
This study was undertaken to determine the influences of various doses of peppermint oil on the hepatic enzymes, alanine transaminase, apartate tranaminase, alkaline phosphotase and gamma glutamyl transferase and the level of malondialdehyde in the serum
Mansourian, A.R.   +3 more
core   +2 more sources

Amplification‐Free, Sequencing‐Free, Detection of Viral RNAs with Variant Specification by Discrete Nanocounting

open access: yesAdvanced Functional Materials, EarlyView.
Integrating selective RNA shortcut, targeted gene isolation, and micro‐arrayed nanoimaging, the NanoPick‐array platform excels in detecting severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) at just 60 copies µL−1 and accurately differentiating variants at the single‐nucleotide level. It ensures 100% accuracy for samples with cycle threshold (
Zixun Wang   +9 more
wiley   +1 more source

Immobilization of Urease onto Nanochitosan Enhanced the Enzyme Efficiency: Biophysical Studies and in Vitro Clinical Application on Nephropathy Diabetic Iraqi Patients

open access: yesJournal of Nanotechnology, 2022
Immobilization of enzymes is an effective method for improving the properties and applications of modern enzymes. There are several supports for enzyme immobilization.
Zahraa S. Al-Garawi   +3 more
doaj   +1 more source

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