Results 51 to 60 of about 59,667 (304)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Immobilization of papain on chitin and chitosan and recycling of soluble enzyme for deflocculation of saccharomyces cerevisiae from bioethanol distilleries [PDF]

, 2015
Yeast flocculation (Saccharomyces cerevisiae) is one of the most important problems in fuel ethanol production. Yeast flocculation causes operational difficulties and increase in the ethanol cost.
Silva, Douglas Fernandes, Carvalho, Ana Flavia Azevedo, Oliva Neto, Pedro de [UNESP], Oliva Neto, Pedro de, Silva, Douglas Fernandes [UNESP], Rosa, Henrique, Rosa, Henrique [UNESP], Oliva-Neto, Pedro   +7 more
core   +1 more source

Iron homeostasis disruption and lipid peroxidation in skeletal muscle during short‐term immobilization

FEBS Open Bio, EarlyView.
14‐day casting‐induced immobilization reduced gastrocnemius muscle mass and increased non‐heme iron and ferritin heavy chain levels. Despite iron accumulation, transferrin receptor 1 and iron regulatory protein 2 were paradoxically upregulated. Lipid peroxidation was elevated without compensatory antioxidant responses.
Haruka Yokogawa, Kazuhiko Higashida, Naoya Nakai   +2 more
wiley   +1 more source

Immobilization and characterization of recombinant esterase enzyme on chitosan nanoparticles [PDF]

, 2011
Immobilization of biologically important molecules on myriad nano-sized materials has attracted great attention. Through this study, thermophilic esterase enzyme was obtained using recombinant DNA technology and purified applying one-step His-Select HF ...
Şanlı Mohamed, Gülşah, Şanli-Mohameda, Gülşa, Sanli-Mohamed, Gulsah, Ilgü, Hüseyin, Turan, Taylan   +4 more
core   +1 more source

Process Optimization and Application of Lipase Immobilized on Phenol Amino Modified MCM-41

Shipin gongye ke-ji
In order to construct a new catalytic system of immobilized lipase and improve the stability of enzyme, the phenol amino modified ordered mesoporous material (MCM-41) was introduced for the study of lipase immobilization.
Qian LI, Xiangbing ZENG, Xitong SUN, Xiaoyi CHEN, Miao LI, Tianyu WANG, Fan HUANG, Fanghong SUN, Xianzhen LI   +8 more
doaj   +1 more source

A new approach to evaluate immobilization of β-galactosidase on Eupergit® C: structural, kinetic, and thermal characterization

Química Nova, 2014
This study aimed to evaluate β-galactosidase immobilization. For this purpose, the ionic strength of the buffer, reaction time, amount of the immobilization support, and pH were evaluated by a central composite design. Assay 8, which consisted of 1.5 mol
Anna R. C. Braga, Marceli F. Silva, José V. Oliveira, Helen Treichel, Susana J. Kalil   +4 more
doaj   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Hidrólise da lactose e síntese de galactooligossacarídeos utilizando B-galactosidase imobilizada em suportes à base de quitosana [PDF]

, 2014
Tese (doutorado) - Universidade Federal de Santa Catarina, Centro Tecnológico, Programa de Pós-Graduação em Engenharia de Alimentos, Florianópolis, 2014.A ß-galactosidase (E.C 3.2.1.23) é uma das enzimas mais empregadas na indústria de alimentos sendo ...
Klein, Manuela Poletto
core  

A Review of Enzyme Immobilization Methods and Critical Parameters for Evaluating Immobilized Enzymes [PDF]

Journal of Mazandaran University of Medical Sciences
‌Enzymes, natural biocatalysts with high catalytic efficiency, are essential in numerous biological and industrial processes. However, their inherent instability and sensitivity to environmental conditions often limit their direct application.
Mehdi Aghaee, Mehdi Mogharabi-Manzari
doaj  

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source