Peptide-modified surfaces for enzyme immobilization. [PDF]
PLoS ONE, 2011 BACKGROUND: Chemistry and particularly enzymology at surfaces is a topic of rapidly growing interest, both in terms of its role in biological systems and its application in biocatalysis.
Jinglin Fu +2 more
doaj +1 more source
Carbon Nanotubes as Supports for Inulinase Immobilization
Molecules, 2014 The commercial inulinase obtained from Aspergillus niger was non-covalently immobilized on multiwalled carbon nanotubes (MWNT-COOH). The immobilization conditions for the carbon nanotubes were defined by the central composite rotational design (CCRD ...
Tais B. Garlet +6 more
doaj +1 more source
Hydrophobic adsorption and covalent immobilization of Candida antarctica lipase B on mixed-function-grafted silica gel supports for continuous-flow biotransformations [PDF]
, 2013 Adsorption onto solid supports has proven to be an easy and effective way to improve the mechanical and catalytic properties of lipases. Covalent binding of lipases onto the support surface enhances the active lifetime of the immobilized biocatalysts ...
Poppe, László +8 more
core +1 more source
Molecules, 2016 This paper describes the immobilization of the neutral protease from Bacillus subtilis and its application in the regioselective hydrolysis of acetylated nucleosides, including building blocks useful for the preparation of anticancer products.
Teodora Bavaro +5 more
doaj +1 more source
Bioorganically doped sol-gel materials containing amyloglucosidase activity [PDF]
Acta Periodica Technologica, 2006 Amyloglucosidase (AMG) from Aspergillus niger was encapsulated in various matrices derived from tetraethoxysilane, methyltriethoxysilane, phenyltriethoxysilane and vinyltriacetoxysilane by different methods of immobilization.
Vlad-Oros Beatrice +4 more
doaj +1 more source
FEBS Letters, EarlyView.We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee +3 more
wiley +1 more source
Immobilization of Cow Rumen Fluid Cellulase by Entrapment in Calcium Alginate Beads [PDF]
, 2012 Cellulases represent a class of enzyme which provide a key opportunity for achieving tremendous benefits of biomass utilization. Cellulase can be produced by fungi, bacteria or Actinomycetes.
Indah , Hartati +2 more
core
Peptide-Mediated Immobilization on Magnetoferritin for Enzyme Recycling
Nanomaterials, 2019 Ferritin possess favorable properties because its exterior and interior surface can be applied to generate functional nanomaterials, which make them possible for enzyme immobilization and recycling.
Yu Zhang +5 more
doaj +1 more source
Immobilization of β-galactosidase onto functionalized graphene nano-sheets using response surface methodology and its analytical applications. [PDF]
PLoS ONE, 2012 β-Galactosidase is a vital enzyme with diverse application in molecular biology and industries. It was covalently attached onto functionalized graphene nano-sheets for various analytical applications based on lactose reduction.Response surface ...
Devesh Kishore +3 more
doaj +1 more source
Calpain small subunit homodimerization is robust and calcium‐independent
FEBS Letters, EarlyView.Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy +4 more
wiley +1 more source