Immobilization of β-galactosidase onto functionalized graphene nano-sheets using response surface methodology and its analytical applications. [PDF]
PLoS ONE, 2012 β-Galactosidase is a vital enzyme with diverse application in molecular biology and industries. It was covalently attached onto functionalized graphene nano-sheets for various analytical applications based on lactose reduction.Response surface ...
Devesh Kishore +3 more
doaj +1 more source
Properties and stabilization of an extracellular α-glucosidase from the extremely thermophilic archaebacteria Thermococcus strain AN 1: enzyme activity at 130°C [PDF]
, 1996 An extracellular α-glucosidase from the thermophilic archaebacterium Thermococcus strain AN1 was purified 875-fold in five steps (Hiload Q-Sepharose, phenyl Sepharose, HPHT-hydroxyapatite, gel filtration and Mono Q chromatography) with a yield of 4%.
Daniel, Roy M. +2 more
core +2 more sources
Peptide-Mediated Immobilization on Magnetoferritin for Enzyme Recycling
Nanomaterials, 2019 Ferritin possess favorable properties because its exterior and interior surface can be applied to generate functional nanomaterials, which make them possible for enzyme immobilization and recycling.
Yu Zhang +5 more
doaj +1 more source
Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation
FEBS Letters, EarlyView.Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe +3 more
wiley +1 more source
Effects of detergents on activity, thermostability and aggregation of immobilized lipases [PDF]
, 2014 Predmetom tejto diplomovej práce bolo štúdium vplyvu detergentov na aktivitu, termostabilitu a agregáciu voľnej a imobilizovanej formy komerčného preparátu lipázy izolovanej z mikroskopickej huby Rhizopus arrhizus. Teoretická časť obsahuje ucelenú rešerš
Bančáková, Anna
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Comparison between a direct-flow SPR immunosensor for ampicillin and a competitive conventional amperometric device: analytical features and possible applications to real samples [PDF]
, 2017 In this research, we developed a direct-flow surface plasmon resonance (SPR) immunosensor for ampicillin to perform direct, simple, and fast measurements of this important antibiotic.
Campanella, Luigi +6 more
core +2 more sources
FEBS Letters, EarlyView.We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee +3 more
wiley +1 more source
Recent advances in enzyme immobilization technology
Xi'an Gongcheng Daxue xuebaoAs an efficient and safe biocatalyst, enzymes have been widely used in many fields such as food, medicine and environmental governance, but it is difficult to realize further industrial application due to the poor environmental stability of free enzymes.
PAN Hong +3 more
doaj +1 more source
Journal of Materials Research and Technology, 2019 In recent years, metal–organic frameworks (MOFs) have received accelerating research attention as a versatile carrier and promising bio-immobilization support materials for enzyme immobilization. This is particularly due to their extraordinary structural
Muhammad Bilal +3 more
doaj +1 more source
Ultrafiltration Fibers like Bioreactors [PDF]
, 2001 A polysulfone ultrafiltration membrane with pectinase physically immobilized on it by a dynamic formation method was used to examine the potential of these reactive membranes in applications involving solutions containing pectin.
Carrin, Maria Elena +2 more
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