Results 171 to 180 of about 308,992 (299)

Viral Infection‐Inspired Autonomous Detection of Fusion‐Competent Viruses for Screening and Environmental Surveillance

Advanced Materials, EarlyView.
Inspired by viral entry mechanisms, the FUSION assay enables autonomous detection of respiratory viruses via membrane fusion–triggered CRISPR‐Cas13a activation. VEACON selectively fuses with fusion‐competent viruses, triggering fluorescence within confined vesicles.
Jae Chul Park, Yidam Song, Hyun‐Woo Choi, Jaeuk Sung, Harin Jin, WonSeok Gwak, Kirim Yoo, Soohwang Lee, Jongeon Park, Jeongmin Kim, Hye‐Jun Jo, Jahyun Koo, Youngdo Jeong, Kwan Hyi Lee, Seung‐Jung Kee, Hojun Kim   +15 more
wiley   +1 more source

Probing the modulation of enzyme kinetics by multi-temperature, time-resolved serial crystallography. [PDF]

Nat Commun
Schulz EC, Prester A, von Stetten D, Gore G, Hatton CE, Bartels K, Leimkohl JP, Schikora H, Ginn HM, Tellkamp F, Mehrabi P.   +10 more
europepmc   +1 more source

High-Throughput Steady-State Enzyme Kinetics Measured in a Parallel Droplet Generation and Absorbance Detection Platform. [PDF]

Anal Chem, 2022
Neun S, van Vliet L, Hollfelder F, Gielen F.   +3 more
europepmc   +1 more source

Stable Protein‐Based G‐Quadruplex‐Derived Supramolecular Bioinks as Tunable ECM‐Mimetic Constructs Assembled by Combining Non‐Covalent and Covalent Strategies

Advanced Materials, EarlyView.
Harnessing the synergistic interplay of supramolecular self‐assembly, under macromolecular crowding conditions, and enzymatic‐mediated covalent crosslinking toward a stable protein‐based G‐quadruplex‐derived supramolecular bioink. This bioinspired strategy enables the biofabrication of complex and tunable ECM‐mimetic constructs, providing a platform ...
Vera Sousa, Rita Sobreiro‐Almeida, Bart W. L. van den Bersselaar, E. W. Meijer, Ghislaine Vantomme, João Borges, João F. Mano   +6 more
wiley   +1 more source

Electrochemical Investigation of Enzyme Kinetics with an Unmediated, Unmodified Platinum Microelectrode: The Case of Glucose Oxidase. [PDF]

ACS Electrochem
Davis C, Fridriksdottir A, Alkhairi O, Sepunaru L.   +3 more
europepmc   +1 more source

Enzyme kinetics by GH7 cellobiohydrolases on chromogenic substrates is dictated by non-productive binding: insights from crystal structures and MD simulation. [PDF]

FEBS J, 2023
Haataja T, Gado JE, Nutt A, Anderson NT, Nilsson M, Momeni MH, Isaksson R, Väljamäe P, Johansson G, Payne CM, Ståhlberg J.   +10 more
europepmc   +1 more source

In Vivo Monitoring of Thrombo‐Inflammatory Biomarkers via Molecularly Imprinted Polymer‐Integrated Hydrogel Microneedles

Advanced Materials, EarlyView.
A wearable electrochemical microneedle patch integrates Prussian Blue redox transduction with molecularly imprinted polymer recognition for reagent‐free sampling and detection of thrombo‐inflammatory biomarkers in dermal interstitial fluid. The platform tracks thrombin and inflammatory cytokines with sensitive in vitro, ex vivo, and in vivo performance,
Mahmoud Ayman Saleh, Peyman GhavamiNejad, Arash Khorrami Jahromi, Sanjana Srikant, Sripadh Guptha Yedire, Fatemeh Keyvani, Roozbeh Siavash Moakhar, Sajad Shiekh, Mackenzie Graham, Joe Quadrilatero, Mahla Poudineh, Sara Mahshid   +11 more
wiley   +1 more source

Responses of maize roots, rhizosphere enzyme kinetics and prokaryote diversity to alternating precipitation: insights from a three-year field study. [PDF]

Ann Bot
Würsig H, Yim B, Martín Roldán M, Ghaderi N, Stoll F, Bouffaud ML, Vetterlein D, Reitz T, Blagodatskaya E, Smalla K, Tarkka M.   +10 more
europepmc   +1 more source

Direct Synthesis of High‐Valence Protein@UiO‐66 Composites: Linking Crystallization Pathways to Protein Encapsulation

Advanced Materials, EarlyView.
This work reports a direct, biocompatible method to synthesize UiO‐66, enabling one‐step encapsulation of proteins without compromising crystallinity or activity. Using advanced in situ and ex situ techniques, the study reveals that proteins integrate concurrently with MOF growth, forming crystalline protein@UiO‐66 nanoparticles, and provide insight ...
Jesús Cases Díaz, Jana Glatz, Elisa Merced Olivas, Ramon Pons, Joseph P. Patterson, Mónica Giménez‐Marqués   +5 more
wiley   +1 more source

Bypass mechanism of F$_1$-ATPase for asymmetric enzyme kinetics

We discovered novel enzyme kinetics of F$_1$-ATPase, a biomolecular motor that synthesizes and hydrolyzes adenosine triphosphate (ATP), using single-molecule experiments and numerical simulations.
Toyabe, Shoichi, Nakayama, Yohei
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