Results 251 to 260 of about 925,011 (313)
Acetoacetate Decarboxylase. Preparation of the Enzyme*
Biochemistry, 1966 Crystalline acetoacetate decarboxylase can be prepared from Clostridium acetobutylicum by the following steps: Preparation of an acetone powder from the bacteria, extraction of this powder at pH 5.9, precipitation of the enzyme at pH 3.8, a single fractionation with ammonium sulfate, and chromatography over diethylaminoethylcellulose, followed by ...
Zerner, Burt +4 more
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HEPARINASE: III. PREPARATION AND PROPERTIES OF THE ENZYME
Canadian Journal of Biochemistry and Physiology, 1956 The extraction of heparinase from beef and rabbit liver has been studied. Optimal conditions for precipitation of the enzyme were 33% ammonium sulphate, pH 7.0; 7% ethanol, pH 5.0–5.5; 40–70% acetone, pH 8.0; with 0.1% NaCl for ethanol and acetone. A new method of preparation of the enzyme is described, using extraction with 0.15 M KCl, precipitation ...
M H, CHO, L B, JAQUES
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Enzymic Activities of Commercial Preparations of Pancreatic Enzymes
Scottish Medical Journal, 1977The enzymic activities of 20 commercial pancreatic extracts have been measured by standard (F.I.P.) methods of assay and the activities have been compared with a standard preparation of pancreatin supplied by the British Pharmacopoeia Commission. A wide range of enzymic activities was found.
S, Drummond +3 more
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Preparation of enzyme calibration materials
Clinica Chimica Acta, 1998Standardisation in clinical enzymology needs not only reference methods but also reference materials. While single-enzyme reference enzymes have been developed, a multienzyme certified reference material (MECRM) available in high amount remains to be produced.
G, Férard, J M, Lessinger
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Use of genetically prepared enzyme conjugates in enzyme immunoassay
Trends in Biochemical Sciences, 1993Enzyme immunoassay, using enzymes crosslinked to either antibodies or antigens, has proved a valuable immunological tool for many years. Recently, gene fusion techniques have been used to prepare these enzyme conjugates. This method may be especially advantageous in cases where (1) the antigen is difficult and costly to obtain in large quantities or (2)
C, Lindbladh, K, Mosbach, L, Bülow
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The Enzymic Preparation of Diphosphoinositides
Preparative Biochemistry, 1977A procedure for the preparation of diphosphoinositides is described. Triphosphoinositides isolated from bovine brain are hydrolysed by the triphosphoinositide phosphatase (EC 3.1.3.36) from Crithidia fasciculata in the presence of MgC12 and cetyltrimethyl-ammonium bromide.
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