Enzyme Stability in Nanoparticle Preparations Part 1: Bovine Serum Albumin Improves Enzyme Function [PDF]
Molecules, 2020 Enzymes have gained attention for their role in numerous disease states, calling for research for their efficient delivery. Loading enzymes into polymeric nanoparticles to improve biodistribution, stability, and targeting in vivo has led the field with ...
Jason Thomas Duskey +6 more
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Glycosylation increases active site rigidity leading to improved enzyme stability and turnover. [PDF]
FEBS J, 2023 Glycosylation is the most prevalent protein post‐translational modification, with a quarter of glycosylated proteins having enzymatic properties. Yet, the full impact of glycosylation on the protein structure–function relationship, especially in enzymes,
Ramakrishnan K +12 more
europepmc +2 more sources
Immobilization of Thiol-Modified Horseradish Peroxidase on Gold Nanoparticles Enhances Enzyme Stability and Prevents Proteolytic Digestion. [PDF]
LangmuirThe specificity and efficiency of enzyme-mediated reactions have the potential to positively impact many biotechnologies; however, many enzymes are easily degraded. Immobilization on a solid support has recently been explored to improve enzyme stability.
Breausche FE +5 more
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Journal of Hazardous Materials, 2022 Laccase has received extensive attention in pollutant degradation due to its high efficiency and environmental friendliness, but free laccase has poor stability, easy inactivation, and difficulty in recycling, which limited its application.
Xueping Li +5 more
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Immobilisation of α-amylase on activated amidrazone acrylic fabric: a new approach for the enhancement of enzyme stability and reusability. [PDF]
Sci Rep, 2019 In this study, amidrazone acrylic fabric was applied as an immobilising support for α-amylase. The immobilised α-amylase was characterised by Fourier transform infrared spectroscopy and scanning electron microscopy.
Al-Najada AR +8 more
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Increasing Enzyme Stability and Activity through Hydrogen Bond-Enhanced Halogen Bonds. [PDF]
Biochemistry, 2018 The construction of more stable proteins is important in biomolecular engineering, particularly in the design of biologics-based therapeutics. We show here that replacing the tyrosine at position 18 (Y18) of T4 lysozyme with the unnatural amino acid m ...
Carlsson AC +6 more
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Enzyme Stability-Activity Trade-Off: New Insights from Protein Stability Weaknesses and Evolutionary Conservation [PDF]
bioRxiv, 2023 A general limitation of the use of enzymes in biotechnological processes under sometimes non-physiological conditions is the complex interplay between two key quantities, enzyme activity and stability, where the increase of one is often associated with ...
Qingzhen Hou +2 more
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The combined effects of reactant kinetics and enzyme stability explain the temperature dependence of metabolic rates. [PDF]
Ecol Evol, 2017 A mechanistic understanding of the response of metabolic rate to temperature is essential for understanding thermal ecology and metabolic adaptation. Although the Arrhenius equation has been used to describe the effects of temperature on reaction rates ...
DeLong JP +6 more
europepmc +2 more sources
Bioinspired Framework Catalysts: From Enzyme Immobilization to Biomimetic Catalysis
Chemical Reviews, 2023 Enzymatic catalysis has fueled considerable interest from chemists due to its high efficiency and selectivity. However, the structural complexity and vulnerability hamper the application potentials of enzymes.
Kun-Yu Wang, Jiandong Pang, Wei Shi
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Aqueous ionic liquids redistribute local enzyme stability via long-range perturbation pathways. [PDF]
Comput Struct Biotechnol J, 2021 El Harrar T +5 more
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