Results 251 to 260 of about 978,452 (311)

Enzyme stability in downstream processing part 1: Enzyme inactivation, stability and stabilization

Biotechnology Advances, 1992
In biotechnological recovery processes the instability of the product can lead to large losses in the sequence of recovery processes needed to purify the product. As the cost of the final active product is strongly dependent on the recovery yield, this will lead to an increase in product cost.
Weijers, S.R., van 't Riet, K.
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Engineering enzymes for stability

Current Opinion in Structural Biology, 1996
There have been many recent developments in elaborating the approaches for stabilizing enzymes by stabilizing the folding state, destabilizing the unfolded state and altering the kinetics of unfolding. However, these represent a series of rules of thumb rather than the reliable principles that would be expected of 'engineering'.
A, Shaw, R, Bott
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The enzyme stability of dehydropeptides

Biochemical and Biophysical Research Communications, 1978
Abstract A pentapeptide, Z-Gly-Gly-Phe-Phe-Ala · OH (1b) and the corresponding unsaturated pentapeptide, Z-Gly-Gly-Phe-Δ Z Phe-Ala · OH (1a) , have been synthesized. The saturated compound (1b) was rapidly hydrolyzed by both chymotrypsin and thermolysin to the expected products, but the dehydropeptide was completely unhydrolyzed by ...
M L, English, C H, Stammer
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Enzyme Stabilization via Cross-Linked Enzyme Aggregates

2010
Extensive cross-linking of a precipitate of a protein by a cross-linking reagent (glutaraldehyde has been most commonly used) creates an insoluble enzyme preparation called cross-linked enzyme aggregates (CLEAs). CLEAs show high stability and performance in both conventional aqueous media as well as nonaqueous media.
Munishwar N, Gupta, Smita, Raghava
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Rational engineering of enzyme stability

Journal of Biotechnology, 2004
During the past 15 years there has been a continuous flow of reports describing proteins stabilized by the introduction of mutations. These reports span a period from pioneering rational design work on small enzymes such as T4 lysozyme and barnase to protein design, and directed evolution.
Eijsink, V.G., Bjork, A., Gaseidnes, S., Sirevag, R., Synstad, B., van der Burg, B., Vriend, G., Vriend, G.   +7 more
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Directed evolution of enzyme stability

Biomolecular Engineering, 2005
Modern enzyme development relies to an increasing extent on strategies based on diversity generation followed by screening for variants with optimised properties. In principle, these directed evolution strategies might be used for optimising any enzyme property, which can be screened for in an economically feasible way, even if the molecular basis of ...
Vincent G H, Eijsink, Sigrid, Gåseidnes, Torben V, Borchert, Bertus, van den Burg   +3 more
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Nanostructures for enzyme stabilization

Chemical Engineering Science, 2006
Recent breakthroughs in nanotechnology have made various nanostructured materials more affordable for a broader range of applications. Although we are still at the beginning of exploring the use of these materials for biocatalysis, various nanostructures have been examined as hosts for enzyme immobilization via approaches including enzyme adsorption ...
Jungbae Kim, Jay W. Grate, Ping Wang
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Enzyme Stabilization by Directed Evolution

2000
Engineered enzymes are widely used in industrial processes, where they provide distinct advantages over the naturally-occurring ones (1, 2). Engineering a useful enzyme is particularly difficult when the target functions—for example, the environment in which it must work or the substrates it must accept--differ significantly from the natural ones ...
Gershenson, Anne, Arnold, Frances H.
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Enzyme stabilization without carriers

Enzyme and Microbial Technology, 1979
Abstract This review deals with the problem of enzyme stabilization without the use of carriers. The following main types of stabilization are considered: addition of low-molecular weight compounds; addition of organic solvents; chemical modification of key functional groups on enzymes; intramolecular crosslinking with bifunctional reagents.
V.P. Torchilin, K. Martinek
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Stabilization of Enzymes by Using Thermophiles

2023
Manufactured steroid compounds have many applications in the pharmaceutical industry. Due to the chemical complexity and chirality of steroids, there is an increasing demand for enzyme-based bioconversion processes to replace those based on chemical synthesis.
Ana-Luisa, Ribeiro, Mercedes, Sánchez, Sandra, Bosch, José, Berenguer, Aurelio, Hidalgo   +4 more
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