Results 31 to 40 of about 5,877,636 (396)

Practical insights on enzyme stabilization [PDF]

Critical Reviews in Biotechnology, 2017
Enzymes are efficient catalysts designed by nature to work in physiological environments of living systems. The best operational conditions to access and convert substrates at the industrial level are different from nature and normally extreme. Strategies to isolate enzymes from extremophiles can redefine new operational conditions, however not always ...
Silva, Carla, Sá, M., Jing Su, Fu, Jiajia, Cavaco-Paulo, Artur   +4 more
openaire   +3 more sources

Effects of single mutations on the stability of horseradish peroxidase to hydrogen peroxide [PDF]

, 2007
Horseradish peroxidase (HRP) is a commonly used enzyme in many biotechnological fields. Improvement of HRP stability would further increase its potential application range.
Arnold, Barry J. Ryan, Bateman, Borole, Ciarán Ó'Fágáin, Hernández-Ruiz, Hiner, Hiner, Hiner, Lehmann, Lei, Maniatis, Miyazaki, O'Brien, Ryan, Schmidt, Valderrama, Wang, Wanger   +18 more
core   +2 more sources

Effect of Water Content on the Thermal Inactivation Kinetics of Horseradish Peroxidase Freeze-Dried from Alkaline pH [PDF]

, 2001
The thermal inactivation of horseradish peroxidase freeze-dried from solutions of different pH (8, 10 and 11.5, measured at 25 C) and equilibrated to different water contents was studied in the temperature range from 110 to 150 C.
Hägerdal B., J. A. Saraiva, J. C. Oliveira, M. A. Lemos, Pizzocaro F., Van Loey A., Whitaker J. R.   +6 more
core   +2 more sources

Enantiomeric Tartaric Acid Production Using cis-Epoxysuccinate Hydrolase: History and Perspectives

Molecules, 2019
Tartaric acid is an important chiral chemical building block with broad industrial and scientific applications. The enantioselective synthesis of l(+)- and d(−)-tartaric acids has been successfully achieved using bacteria presenting cis ...
Jinsong Xuan, Yingang Feng
doaj   +1 more source

Immobilization of Glucose Oxidase on Eupergit C: Impact of Aeration, Kinetic and Operational Stability Studies of Free and Immobilized Enzyme [PDF]

Chemical and Biochemical Engineering Quarterly, 2019
The effect of aeration on the stability of glucose oxidase in the reaction of glucose oxidation to gluconic acid was investigated by determining the operational stability decay rate constant in the process conditions.
D. Pečar, Đ. Vasić-Rački, A. Vrsalović Presečki   +2 more
doaj   +1 more source

Purification and characterization of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. G1 [PDF]

, 2005
A cyclodextrin glucanotransferase (CGTase) was successively purified by ammonium sulphate precipitation, and affinity chromatography on a-CD (epoxy)-Sepharose 6B column. The specific activity of the CGTase was increased approximately 2200-fold, from 8.43
A. Rahman, R, Hassan, O., Ho, Kek Sian, Ismail, A. F., Kamaruddin, K., Md. Illias, R, Nik Mahmood, N. A., Said, M.   +7 more
core   +1 more source

Activity-Stability Relationships in Extremophilic Enzymes [PDF]

Journal of Biological Chemistry, 2003
Psychrophilic, mesophilic, and thermophilic alpha-amylases have been studied as regards their conformational stability, heat inactivation, irreversible unfolding, activation parameters of the reaction, properties of the enzyme in complex with a transition state analog, and structural permeability.
D'Amico, Salvino, Marx, J. C., Gerday, Charles, Feller, Georges   +3 more
openaire   +2 more sources

Graphene Oxide Nanosheets Shielding of Lipase Immobilized on Magnetic Composites for the Improvement of Enzyme Stability

ACS Sustainable Chemistry and Engineering, 2019
In this study, a novel enzyme immobilization method was developed to enhance the catalytic stability of enzymes. In this strategy, ionic liquid (IL) modified magnetic chitosan (MCS) composites were used as supports for lipase adsorption and graphene ...
Hongbo Suo, Lili Xu, Chaodi Xu, X. Qiu, Hongyue Chen, He Huang, Yi Hu   +6 more
semanticscholar   +1 more source

Consensus mutagenesis reveals that non-helical regions influence thermal stability of horseradish peroxidase [PDF]

, 2008
The enzyme horseradish peroxidase has many uses in biotechnology but a stabilized derivative would have even wider applicability. To enhance thermal stability, we applied consensus mutagenesis (used successfully with other proteins) to recombinant ...
Amin, Arnold, Azevedo, Barry J. Ryan, Bonnaffe, Carlsson, Ciarán Ó'Fágáin, Dunford, Duroux, Eijsink, Eremin, Fishman, Flores, French, Gajhede, Garcia, Gonera, Grigorenko, Ignatenko, Kamal, Kamal, Kuhlmeyer, Lehmann, Lehmann, Lei, Lesk, Lin, Lipovsek, Liu, Magliery, Maniatis, Mary J. O'Connell, McEldoon, Miland, Miland, Miyazaki, Morawski, Nielsen, O'Brien, O'Brien, Pina, Ryan, Ryan, Ryan, Ryan, Ryan, Smith, Steipe, Tams, Thompson, van den Burg, Veitch, Wang, Watanabe, Welinder, Yang, Yang   +56 more
core   +1 more source

The development of human sera tests for HDL-bound serum PON1 and its lipolactonase activitys⃞

Journal of Lipid Research, 2007
Serum paraoxonase (PON1) is a lipolactonase that associates with HDL-apolipoprotein A-I (HDL-apoA-I) and thereby plays a role in the prevention of atherosclerosis. Current sera tests make use of promiscuous substrates and provide no indications regarding
Leonid Gaidukov, Dan S. Tawfik
doaj   +1 more source