Results 61 to 70 of about 978,452 (311)

Simplified immobilisation method for histidine-tagged enzymes in poly(methyl methacrylate) microfluidic devices [PDF]

, 2017
Article in press. Kulsharova, G., New BIOTECHNOLOGY (2017), https://doi.org/10.1016/j.nbt.2017.12.004Poly(methyl methacrylate) (PMMA) microfluidic devices have become promising platforms for a wide range of applications.
Baganz, Frank, Dimov, Nikolay, Kulsharova, Gulsim, Marques, Marco P.C., Szita, Nicolas   +4 more
core   +3 more sources

Function‐driven design of a surrogate interleukin‐2 receptor ligand

FEBS Letters, EarlyView.
Interleukin (IL)‐2 signaling can be achieved and precisely fine‐tuned through the affinity, distance, and orientation of the heterodimeric receptors with their ligands. We designed a biased IL‐2 surrogate ligand that selectively promotes effector T and natural killer cell activation and differentiation. Interleukin (IL) receptors play a pivotal role in
Ziwei Tang, Zelin Cheng, Teng Li, Fulian Wang, Liangminghui Zhang, Xiuxiu He, Lili Liu, Wei Wang, Aibin Liang, Guang Yang   +9 more
wiley   +1 more source

Characterization of thermophilic β-Glucosidase of rhizospheric bacterial strain (LSKB15) isolated from Cholistan Desert, Pakistan [PDF]

, 2018
Fifty thermophilic bacterial strains isolated from rhizospheric soil of Cholistan desert, Pakistan, and designated as LSKB01-LSKB50 were screened for β-glucosidase gene (bgl) belonging to glycoside hydrolase family 1 (GH 1) using PCR technique ...
Ahmed, Amer, Batool, Kashfa, Bibi, Aasia, Rafi, Sarwat, ul Hassan Nasim, Faiz   +4 more
core  

Reclamation of Marine Chitinous Materials for Chitosanase Production via Microbial Conversion by Paenibacillus macerans [PDF]

, 2018
[[abstract]]: Chitinous materials from marine byproducts elicit great interest among biotechnologists for their potential biomedical or agricultural applications.
Anh Dzung Nguyen, Chien Thang Doan, San-Lang Wang, Thi Ngoc Tran, Van Bon Nguyen   +4 more
core   +1 more source

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

Crowding Promotes the Switch from Hairpin to Pseudoknot Conformation in Human Telomerase RNA

, 2011
Formation of a pseudoknot in the conserved RNA core domain in the ribonucleoprotein human telomerase is required for function. In vitro experiments show that the pseudoknot (PK) is in equilibrium with an extended hairpin (HP) structure.
Antal M., Asakura S., Blasco M. A., Bloomfield V. A., Cao S., Cao S., Chen J. L., Cheung M. S., Comolli L. R., D. Thirumalai, Denesyuk N. A., Dhar A., Feng J., Gilley D., Hyeon C., Kilburn D., Kirkpatrick T. R., Leontis N. B., Lin J. C., McGuffee S. R., Montange R. K., Natalia A. Denesyuk, Pincus D. L., Shaw M. R., Theimer C. A., Theimer C. A., Thirumalai D., Walter H., Whitford P. C., Xia T., Zhou H.-X.   +30 more
core   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

Catalytic Properties of Lipase Extracts from Aspergillus niger

Food Technology and Biotechnology, 2006
Screening of lipolytic strains using Rhodamine-B/olive oil plate technique allowed the selection of Aspergillus niger MYA 135. Lipase production in submerged culture containing 2 % olive oil was enhanced by more than 50 % compared to basal cultural ...
Cintia M. Romero, Mario D. Baigori, Guillermo R. Castro, Licia M. Pera   +3 more
doaj  

Production and characterization of endo-polygalacturonase from Aspergillus niger in solid-state fermentation in double-surface bioreactor

Brazilian Archives of Biology and Technology, 2011
Endo-polygalacturonase (endo-PG) production by Aspergillus niger T0005/007-2 in solid medium with 170 mm of height was evaluated in a cylindrical double surface bioreactor in 96-h experiments.
Diogo Henrique Hendges, Queli Montanari, Eloane Malvessi, Mauricio Moura da Silveira   +3 more
doaj   +1 more source

Immobilization of Isocitrate Dehydrogenase on Mesoporous Silica Foam for Carbon Dioxide Capture [PDF]

E3S Web of Conferences, 2019
Carbon capture can be realized effectively through isocitrate dehydrogenase reaction and the reaction rate was strongly affected by the environmental parameters such as pH and temperature.
Xia Shunxiang, Zhang Laibao, Veony Enjelia   +2 more
doaj   +1 more source