Results 111 to 120 of about 161,575 (283)

Chemically stabilized subtilisins in peptide synthesis [PDF]

open access: yes, 2008
We have stabilized alcalaseTM and subtilisin Carlsberg (SC) against heat by chemical modification with ethylene glycol bis-succinimidyl succinate (EGNHS), a procedure not previously reported for subtilisins.
Colleary, Sandra, Ó Fágáin, Ciarán
core  

Dual‐Ligand Metal‐Organic Frameworks via In Situ Amidoxime Engineering for Selective Ion Separation

open access: yesAdvanced Functional Materials, EarlyView.
Inspired by microbial ion‐trapping mechanisms, a mild and universal strategy is developed to construct highly porous amidoxime‐functionalized MOFs. DFT calculations and molecular force measurements reveal that the dual‐ligand amidoxime configuration significantly strengthens Ga(III) affinity.
Zhifang Lv   +9 more
wiley   +1 more source

A novel hydrogen peroxide biosensor based on modified electrode with hemoglobin and zinc oxide nanoparticles [PDF]

open access: yes, 2012
In this study, direct electron transfer between immobilized hemoglobin (Hb) and zinc oxide nanoparticles modified carbon paste electrode was studied. Direct electrochemical response of Hb on the modified electrode can be achieved and a couple of well ...
Arabnezhad-Khanooki, T.   +7 more
core  

Editorial: Special Issue — Enzyme Immobilization [PDF]

open access: yesMolecules, 2014
Immobilization of enzymes and proteins is a requirement for many industrial enzyme applications, as this facilitates enzyme recovery and reuse. Bearing in mind this necessity, the coupling of immobilization to the improvement of other enzyme features has been pursued by many researchers, and nowadays immobilization is recognized as a tool to improve ...
openaire   +4 more sources

Unraveling the Heterogeneity of Cargo Distribution in the Exogenous Association of Proteins With Extracellular Vesicles

open access: yesAdvanced Healthcare Materials, EarlyView.
We characterized the distribution of cargo proteins associated with extracellular vesicles using various exogenous loading methods. In all cases, single‐particle analysis revealed that the distribution of protein content per EV is heterogeneous, following an exponential decay function.
Karl Normak   +6 more
wiley   +1 more source

Immobilization of Cellulase for Industrial Production

open access: yesChemical Engineering Transactions, 2012
Immobilized enzymes are used in analytical chemistry and as catalysts for the production of chemicals, pharmaceuticals and food. Because of their particular structure, immobilized enzymes require optimal conditions, different from those of soluble ...
G. Hojnik Podrepsek   +3 more
doaj   +1 more source

The Science of Enzyme Immobilization

open access: yes, 2020
Protocols for simple immobilization of unstable enzymes are plenty, but the vast majority of them, unfortunately, have not reached their massive implementation for the preparation of improved heterogeneous biocatalyst. In this context, the science of enzyme immobilization demands new protocols capable of fabricating heterogeneous biocatalysts with ...
Guisán, José Manuel   +4 more
openaire   +3 more sources

Coagulative Granular Hydrogels with an Enzyme Catalyzed Fibrin Network for Endogenous Tissue Regeneration

open access: yesAdvanced Healthcare Materials, EarlyView.
Coagulative granular hydrogels are composed of packed thrombin‐functionalized microgels that catalyze the conversion of fibrinogen into a secondary fibrin network, filling the interstitial voids. This bio‐inspired approach stabilizes the biomaterial to match the robustness of bulk hydrogels without compromising injectability, mimicking the initial ...
Zhipeng Deng   +16 more
wiley   +1 more source

Application and mechanisms of biochar-immobilized enzymes in environmental remediation: a review

open access: yesBiochar
As environmental pollution becomes an increasingly severe issue, the technology of enzyme immobilization on biochar has emerged as a promising solution for water and soil pollution remediation due to its efficiency, cost-effectiveness, and environmental ...
Sinan Zhang   +4 more
doaj   +1 more source

Immobilized phosphorylase for synthesis of polysaccharides from glucose [PDF]

open access: yes, 1972
Continuous processes for enzymatic production of carbohydrates from glucose are discussed. Key reactant in process is identified as phosphorylase which catalyzes reversible formation or degradation of polysaccharide.
Marshall, D. L.
core   +1 more source

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