Bioenzymatic Approach to Cholesterol Detection Using Cholesterol Oxidase-Immobilized Copper-Incorporated Zeolitic Imidazolate Framework-8. [PDF]
Mechoor A +4 more
europepmc +1 more source
A radical‐mediated in situ fluorescence dye deposition (RIFD) reaction containing merely three elements of beads, dyes, and radicals is discovered for the first time, and its underlying mechanism is elucidated. This simple RIFD enables the interfacial and ultrafast deposition of free dyes onto barcode beads, serving as a powerful signal amplification ...
Jiayu Zhang +8 more
wiley +1 more source
Enzyme Co-Immobilization on Precipitated Silica for Sustainable Lactobionic Acid Production. [PDF]
Piątek-Gołda W +7 more
europepmc +1 more source
A Universal Protein Ladder for Standardization of Diverse FRET Assays
Translating FRET measurements from in vitro to intracellular environments requires universal benchmarks. We present a modular protein ladder using TPR motifs to harmonize data across diverse platforms. This predictable calibration curve bridges single molecule FRET measurements, with cell based measurements including FLIM‐FRET.
Evelyn R. Smith +5 more
wiley +1 more source
Synergistic Action of Rhizopus oryzae Lipase and Fusarium oxysporum Cutinase in the Enzymatic Transesterification of a High-Free Fatty Acid Oil. [PDF]
Spanou A +3 more
europepmc +1 more source
A rigid‑flexible integrated CO depot is engineered by embedding rigid COFQDs into a flexible ECM gel network. The rigid framework enables high‑capacity CO storage and sonodynamic bacterial killing, while the flexible ECM gel controls sustained CO release, collectively eradicating multidrug‑resistant infection and promoting scarless healing.
Baohong Sun +13 more
wiley +1 more source
Unveiling the Physicochemical Properties of Magnetic Nanoparticles as Solid Carriers for Laccase Immobilization Toward Different Reducing Substrates. [PDF]
Costa J +4 more
europepmc +1 more source
Cancer‐Associated BCL‐2 Mutants Reveal Mechanisms Towards Venetoclax Resistance
Venetoclax (VEN) resistance in chronic lymphocytic leukemia arises from diverse BCL2 mutations. We map mechanisms contributing to VEN resistance across common BCL‐2 variants. G101V and D103Y reduce drug binding and increase sequestration of pro‐apoptotic proteins. V156D blocks VEN allosterically.
Jonas Aufdermauer +9 more
wiley +1 more source
Immobilized Wild-Type and Mutant L‑Alanine Dehydrogenase from <i>Thermus thermophilus</i> Coupled with Formate Dehydrogenase for Asymmetric Synthesis of Noncanonical Amino Acids. [PDF]
Demir Ğ, Yildirim D, Binay B.
europepmc +1 more source
Probing Electrocatalyst Design for Product Selectivity in CO2 Reduction
Selective CO2 electroreduction is controlled by catalyst structure, dynamic surface reconstruction, adsorbate interactions, and reaction microenvironment. This review summarizes Cu‐ and non‐Cu‐based catalysts, single‐atom and molecular systems, degradation pathways, and operando/computational strategies for steering CO2RR toward hydrocarbons ...
Suvodeep Sen +5 more
wiley +1 more source

