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2020
Solid-state NMR has become the method of choice for the assessment of protein structure for insoluble objects lacking long-range order. In this context, it is apparent that solid-state NMR is also perfectly poised toward the characterization of immobilized proteins. For these systems, it is possible to understand at the atomic level which perturbations,
Cerofolini L. +3 more
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Solid-state NMR has become the method of choice for the assessment of protein structure for insoluble objects lacking long-range order. In this context, it is apparent that solid-state NMR is also perfectly poised toward the characterization of immobilized proteins. For these systems, it is possible to understand at the atomic level which perturbations,
Cerofolini L. +3 more
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The Science of Enzyme Immobilization
2020Protocols for simple immobilization of unstable enzymes are plenty, but the vast majority of them, unfortunately, have not reached their massive implementation for the preparation of improved heterogeneous biocatalyst. In this context, the science of enzyme immobilization demands new protocols capable of fabricating heterogeneous biocatalysts with ...
Jose M, Guisan +4 more
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Immobilization of enzymes with polyaziridines
Journal of Biotechnology, 1990A novel method of enzyme immobilization using a low molecular weight prepolymer of tri-functional aziridines which can immobilize enzymes both by covalent attachment and entrapment within a gel matrix is described. The enzymes are immobilized on a solid support and exhibit an excellent retention of enzymatic activity.
L L, Wood +4 more
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Bacteriolysis by immobilized enzymes
Biotechnology and Bioengineering, 1977AbstractBacteriolytic enzymes produced by Achromobacter lunatus were immobilized in collagen membrane. Intact bacteria such as Pseudomonas solanacearum, Xanthomonas oryzae, Staphylococcus aureus, and Pseudomonas aeruginosa were lyzed with the bacteriolytic enzyme–collagen membrane. Relative activity of the bacteriolytic enzyme–collagen membrane against
I, Karube, T, Suganuma, S, Suzuki
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Immobilized Enzymes and Their Applications
2019The main goal of enzyme immobilization is the re-use of enzymes for many reaction cycles and extended periods of time. Many novel strategies were developed during the last 20 years. The most common are thec arrier-bound technologies employing synthetic and natural polymers, but also carrier-free enzyme immobilization using chemical crosslinking ...
Cacicedo, Maximiliano Luis +8 more
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Enzyme immobilization on heparin
Journal of Biomedical Materials Research, 1978AbstractWe describe the preparation and some of the properties of heparin‐bound α‐chymotrypsin that were obtained via activation of heparin with water‐soluble carbodiimide. Immobilized enzyme has unchanged kinetic characteristics toward low‐molecular‐weight and macromolecular substrates.
V P, Torchilin +4 more
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Enzyme immobilization in porous solid supports—penetration of immobilized enzyme
Biotechnology and Bioengineering, 1984AbstractThe process of enzyme immobilization under the diffusion‐controlled regime (i.e., fast attachment of enzyme compared to its diffusion) is modeled and theoretically solved in this article. Simple and compact solutions for the penetration depth of immobilized enzyme and the bulk enzyme concentration versus time are presented.
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Enzyme immobilization on fibrin
Biotechnology and Bioengineering, 1976The following conclusions can be drawn concerning the utilization of fibrin to immobilized enzyme systems. Fibrin can be used both as a powder or membrane, to covalently immobilize trypsin with retention of activity. Carbon-14 labeled trypsin can be used to estimate the amount of immobilized enzyme on a proteinaceous support.
J G, Dillon, C W, Wade, W H, Daly
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Enzyme immobilization on tritylagarose
Biotechnology and Bioengineering, 1982AbstractA method is described for the immobilization on tritylated agarose or Sepharose columns of a wide spectrum of enzymes, including types useful in contemporary biochemistry/molecular biology, many of which have never before been reported as immobilized.
P, Cashion +5 more
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Pellicular immobilized enzymes
Biochimica et Biophysica Acta (BBA) - Enzymology, 1974Abstract Methods for the preparation of shell-structured immobilized enzymes supported by a fluid impervious spherical core are outlined. The preparation of such trypsin resins by coreticulation of the enzyme with a copolymer of maleic anhydride and vinyl methylester in situ on the surface of glass beads is described and some properties of the ...
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