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The development of enzyme immobilization started in the middle of the previous century as a potential answer to the problem of the enzyme recovery and reuse [...]
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Enzyme immobilization: an update [PDF]
Compared to free enzymes in solution, immobilized enzymes are more robust and more resistant to environmental changes. More importantly, the heterogeneity of the immo-bilized enzyme systems allows an easy recovery of both enzymes and products, multiple re-use of enzymes, continuous operation of enzymatic processes, rapid termination of reactions, and ...
A. A. Homaei+3 more
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Glucose oxidase (GOX) and catalase (CAT) were co-immobilized in silica–calcium–alginate hydrogels to degrade must glucose. The effect of the enzyme dose (1.2–2.4 U/mL), the initial must pH (3.6–4.0), and the incubation temperature (10–20 °C) on the ...
David del-Bosque+4 more
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Research Progresses and Application of Biofuel Cells Based on Immobilized Enzymes
Enzymatic biofuel cells (EBFCs) are devices that use natural enzymes as catalysts to convert chemical energy from bio-sourced fuels into electrical energy.
Jian Zhou+4 more
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Chaperonin-Inspired pH Protection by Mesoporous Silica SBA-15 on Myoglobin and Lysozyme [PDF]
While enzymes are valuable tools in many fields of biotechnology, they are fragile and must be protected against denaturing conditions such as unfavorable solution pH.
Coppens, MO, Liu, J, Lynch, MM, Nigra, M
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Co-Immobilization of Tri-Enzymes for the Conversion of Hydroxymethylfurfural to 2,5-Diformylfuran
Acting as a “green” manufacturing route, the enzyme toolbox made up of galactose oxidase, catalase, and horseradish peroxidase can achieve a satisfactory yield of 2,5-diformylfuran derived from 30 mM hydroxymethylfurfural.
Zhuofu Wu+4 more
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Cellulase immobilization on superparamagnetic nanoparticles for reuse in cellulosic biomass conversion [PDF]
Current cellulosic biomass hydrolysis is based on the one-time use of cellulases. Cellulases immobilized on magnetic nanocarriers offer the advantages of magnetic separation and repeated use for continuous hydrolysis. Most immobilization methods focus on
Fernando Segato+5 more
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Obtaining stabilized enzymes and their application in the food industry [PDF]
The article describes the traditional technological method for the production of cognacs and alcoholic beverages with the participation of immobilized enzymes, based on the fact that the resulting immobilized enzymes are stable in an organic environment.
Shukurova, Shakhina+2 more
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The Science of Enzyme Immobilization
Protocols for simple immobilization of unstable enzymes are plenty, but the vast majority of them, unfortunately, have not reached their massive implementation for the preparation of improved heterogeneous biocatalyst. In this context, the science of enzyme immobilization demands new protocols capable of fabricating heterogeneous biocatalysts with ...
Fernando López-Gallego+6 more
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l-Amino Acid Production by a Immobilized Double-Racemase Hydantoinase Process: Improvement and Comparison with a Free Protein System [PDF]
Protein immobilization is proving to be an environmentally friendly strategy for manufacturing biochemicals at high yields and low production costs. This work describes the optimization of the so-called “double-racemase hydantoinase process,” a system of
Clemente Jiménez, María José+3 more
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