Results 81 to 90 of about 161,575 (283)

CRISPR/Cas9‐Assisted Microrobots for Fast and Ultrasensitive “On‐The‐Fly” Next‐Generation DNA Detection

Advanced Functional Materials, EarlyView.
This work presents self‐propelled CRISPR/Cas9‐functionalized Au–MRs for rapid, amplification‐free, “on‐the‐fly” DNA detection. By harnessing motion‐assisted signal recovery, the platform achieved the limit of detection in low fM DNA concentrations, enabling detection across a wide dynamic range within only 5 min, which is significantly faster than any ...
Jyoti, Petr Michálek, Zbyněk Heger, Martin Pumera   +3 more
wiley   +1 more source

Effects of detergents on activity, thermostability and aggregation of immobilized lipases [PDF]

, 2014
Predmetom tejto diplomovej práce bolo štúdium vplyvu detergentov na aktivitu, termostabilitu a agregáciu voľnej a imobilizovanej formy komerčného preparátu lipázy izolovanej z mikroskopickej huby Rhizopus arrhizus. Teoretická časť obsahuje ucelenú rešerš
Bančáková, Anna
core  

Monomeric PcrA helicase processively unwinds plasmid lengths of DNA in the presence of the initiator protein RepD [PDF]

, 2013
The helicase PcrA unwinds DNA during asymmetric replication of plasmids, acting with an initiator protein, in our case RepD. Detailed kinetics of PcrA activity were measured using bulk solution and a single-molecule imaging technique to investigate the ...
Arbore, Arthur, Atkinson, Bird, Brune, Byrd, Byrd, Chao, Cheng, Christopher P. Toseland, Corrie, Dillingham, Dillingham, Dillingham, Dillingham, Dillingham, Fili, Fili, Gorbalenya, Greenstein, Gregory I. Mashanov, Iordanescu, Justin E. Molloy, Kunzelmann, Leatherbarrow, Lee, Levin, Liisa T. Chisty, Lohman, Lohman, Manosas, Mark S. Dillingham, Martin R. Webb, Mashanov, Natalia Fili, Niedziela-Majka, Noirot, Sellers, Singleton, Slatter, Soultanas, Soultanas, Soultanas, Subramanya, Thomas, Thomas, Toseland, Veaute, Velankar, Webb, Yamaguchi, Yang, Yarranton, Yeeles, Zhang   +54 more
core   +1 more source

Microporous Microgel Assemblies Facilitating the Recruitment and Osteogenic Differentiation of Progenitor Cells for Bone Regeneration

Advanced Functional Materials, EarlyView.
There is a significant need for biomaterials with well‐defined stability and bioactivity to support tissue regeneration. In this study, we developed a tunable microgel platform that enables the decoupling of stiffness from porosity, thereby promoting bone regeneration.
Silvia Pravato, Haishuo Guan, Bianca M. Carrara, Mira Jacobs, Matteo Monticelli, Lukas Moser, Thomas Moragues, Andrew J. deMello, Tomaso Zambelli, Martin Ehrbar   +9 more
wiley   +1 more source

Bioremediation of Hazardous Pollutants Using Enzyme-Immobilized Reactors

Molecules
Bioremediation uses the degradation abilities of microorganisms and other organisms to remove harmful pollutants that pollute the natural environment, helping return it to a natural state that is free of harmful substances.
Hiroshi Yamaguchi, Masaya Miyazaki
doaj   +1 more source

Carbon dioxide/methanol conversion cycle based on cascade enzymatic reactions supported on superparamagnetic nanoparticles

Anais da Academia Brasileira de Ciências, 2017
The conversion of carbon dioxide into important industrial feedstock is a subject of growing interest in modern society. A possible way to achieve this goal is by carrying out the CO2/methanol cascade reaction, allowing the recycle of CO2 using either ...
CATERINA G.C. MARQUES NETTO, LEANDRO H. ANDRADE, HENRIQUE E. TOMA   +2 more
doaj   +1 more source

The immobilization of Kluyveromyces fragilis and Saccharomyces cerevisiae in polyacrylamide gel : a thesis presented in partial fulfilment of the requirements for the degree of Master of Technology in Biotechnology at Massey University [PDF]

, 1980
The search for new energy sources has indicated that biomass, in the form of green plant materials and biological wastes, can provide a perpetual energy source if converted to a useful form.
Dillon, Judith Ann
core  

Supraparticles Composed of Graphitic Carbon Nitride Nanoparticles and Silica‐Supported Horseradish Peroxidase as Customizable Hybrid Catalysts for Photo‐Biocatalytic Cascade Reactions in Continuous Flow

Advanced Functional Materials, EarlyView.
Herein presented supraparticles combine the nanoparticulate photocatalyst graphitic carbon nitride with the enzyme horseradish peroxidase, which is immobilized on silica nanoparticles. In an optimized compatibility range, both catalysts operate effectively within the hybrid supraparticles and catalyze a cascade reaction consisting of the photocatalytic
Bettina Herbig, Egzon Cermjani, Doris Hanselmann, Johannes Prieschl, Angelika Schmitt, Michael S. Moritz, Christian Papp, Greta Nölke, Stefano Di Fiore, Christoph Deckers, Thomas H. Rehm, Susanne Wintzheimer   +11 more
wiley   +1 more source

Functional Materials for Environmental Energy Harvesting in Smart Agriculture via Triboelectric Nanogenerators

Advanced Functional Materials, EarlyView.
This review explores functional and responsive materials for triboelectric nanogenerators (TENGs) in sustainable smart agriculture. It examines how particulate contamination and dirt affect charge transfer and efficiency. Environmental challenges and strategies to enhance durability and responsiveness are outlined, including active functional layers ...
Rafael R. A. Silva, Giulio Fatti, Emanuel Carlos, Guilherme Ferreira, Sumita Goswami, Suman Nandy, Luiz H. C. Mattoso, Elvira Fortunato, Caio G. Otoni, Rodrigo Martins   +9 more
wiley   +1 more source

Effect of Cultivation Time and Medium Condition in Production of Bacterial Cellulose Nanofiber for Urease Immobilization

International Journal of Polymer Science, 2015
A new nanoporous biomatrix originated from bacterial resources has been chosen for urease immobilization. Urease has been immobilized on synthesized bacterial cellulose nanofiber since this enzyme has a key role in nitrogen metabolism.
M. Pesaran, Gh. Amoabediny, F. Yazdian
doaj   +1 more source