Results 91 to 100 of about 3,038,812 (378)

Ion channel function of polycystin‐2/polycystin‐1 heteromer revealed by structure‐guided mutagenesis

FEBS Letters, EarlyView.
Mutations in polycystin‐1 (PC1) or polycystin‐2 (PC2) cause autosomal‐dominant polycystic kidney disease (ADPKD). We generated a novel gain‐of‐function PC2/PC1 heteromeric ion channel by mutating pore‐blocking residues. Moreover, we demonstrated that PC2 will preferentially assemble with PC1 to form heteromeric complexes when PC1 is co‐expressed ...
Tobias Staudner, Juthamas Khamseekaew, M. Gregor Madej, Linda Geiges, Bardha Azemi, Christine Ziegler, Christoph Korbmacher, Alexandr V. Ilyaskin   +7 more
wiley   +1 more source

Glycolytic enzymes - novel carbohydrate binding proteins for glycoprotein analysis [PDF]

, 2011
•The cloning, expression, purification and characterisation of recombinant prokaryotic glycolytic enzymes •The mutagenesis of prokaryotic glycolytic enzymes to generate novel recombinant carbohydrate binding proteins •The characterisation of the ...
Clarke, Paul A., Harrington, Amy, O'Connor, Brendan, Thompson, Roisin   +3 more
core  

Dynamic chromatin: concerted nucleosome remodelling and acetylation [PDF]

, 2005
The flexibility of chromatin that enables translation of environmental cues into changes in genome utilisation, relies on a battery of enzymes able to modulate chromatin structure in a highly targeted and regulated manner.
Becker, Peter, Eberharter, Anton, Ferreira, Roger   +2 more
core   +1 more source

Metabolic Optimization by Enzyme-Enzyme and Enzyme-Cytoskeleton Associations [PDF]

, 2012
Probably enzymes are not dispersed in the cytoplasm, but are bound to each other and to specific cytoskeleton proteins. Associations result in substrate channeling from one enzyme to another. Multienzymatic complexes, or metabolons have been detected in glycolysis, the Krebs cycle and oxidative phosphorylation.
Adela Mújica, Antonio Peña, José G. Sampedro, Salvador Uribe-Carvajal, Natalia Chiquete-Félix, Monica Rosas-Lemus, Gisela Ruíz Granados, Daniela Araiza-Olivera   +7 more
openaire   +3 more sources

Decoding the dual role of autophagy in cancer through transcriptional and epigenetic regulation

FEBS Letters, EarlyView.
Transcriptional and epigenetic regulation controls autophagy, which exerts context‐dependent effects on cancer: Autophagy suppresses tumorigenesis by maintaining cellular homeostasis or promotes tumor progression by supporting survival under stress. In this “In a Nutshell” article, we explore the intricate mechanisms of the dual function of autophagy ...
Young Suk Yu, Ik Soo Kim, Sung Hee Baek
wiley   +1 more source

BsuMI regulates DNA transformation in Bacillus subtilis besides the defense system and the constructed strain with BsuMI-absence is applicable as a universal transformation platform for wild-type Bacillus

Microbial Cell Factories
Background To effectively introduce plasmids into Bacillus species and conduct genetic manipulations in Bacillus chassis strains, it is essential to optimize transformation methods.
Zhao Xingya, Fu Xiaoping, Zhen Jie, Yang Jun, Zheng Hongchen, Bai Wenqin, Song Hui   +6 more
doaj   +1 more source

Influence of enzymatic cow milk hydrolysates on IgA and IgG response of Balb/c mice organism

Czech Journal of Food Sciences, 2005
The manuscript presents the application of an animal model, Balb/c mice, in studies aimed at identifying among enzymatic hydrolysates of sodium caseinate and whey proteins the product with the most beneficial impact on the animal organism.
Barbara Wróblewska, Lucjan Jędrychowski   +1 more
doaj   +1 more source

Serum γ-glutamyltransferase, alanine aminotransferase and aspartate aminotransferase activity in healthy blood donor of different ethnic groups in Gorgan [PDF]

, 2016
Introduction: Measure of liver enzymes may help to increase safety of blood donation for both blood donor and recipient. Determination of liver enzymes may prepare valuable clinical information.
Marjani, A., Mehrpouya, M., Pourhashem, Z.   +2 more
core   +1 more source

Autophagy in cancer and protein conformational disorders

FEBS Letters, EarlyView.
Autophagy plays a crucial role in numerous biological processes, including protein and organelle quality control, development, immunity, and metabolism. Hence, dysregulation or mutations in autophagy‐related genes have been implicated in a wide range of human diseases.
Sergio Attanasio
wiley   +1 more source

Single-substrate Enzyme Kinetics: The Quasi-steady-state Approximation and Beyond [PDF]

J. Math. Chem. 51 (2013) 1467, 2012
We analyze the standard model of enzyme-catalyzed reactions at various substrate-enzyme ratios to identify the regions of validity of the quasi-steady-state approximation. Certain prevalent conditions are checked and compared against the actual findings. Efficacies of a few other measures are highlighted. Some very recent observations are rationalized,
arxiv