Results 81 to 90 of about 2,144,314 (302)

Biochemical Characterisation of Reconstructed Ancestral CM-DAH7PS Enzymes [PDF]

, 2012
Thermophily has been proposed to be a primitive trait (Stetter, 2006, Pace, 1991, Woese, 1987) which has led to suggestions that all contemporary thermophilic species are the direct descendants of ancient thermophilic organisms.
McMillan, Joel Patrick
core  

Substrate Recognition Governs Reverse Transcriptase Resistance to Diagnostic Inhibitors in RT-qPCR

Diagnostics
Background: Reverse transcription is a key step in emerging RNA diagnostics, but reverse transcriptase (RT) enzymes often fail in the presence of inhibitors carried over from clinical samples or introduced during RNA extraction.
Inês F. Costa, Vânia O. Fernandes, Victor D. Alves, Virgínia M. R. Pires, Joana A. Brás, Pedro Bule, Carlos M. G. A. Fontes   +6 more
doaj   +1 more source

The Characterization and Modification of a Novel Bifunctional and Robust Alginate Lyase Derived from Marinimicrobium sp. H1

Marine Drugs, 2019
Alginase lyase is an important enzyme for the preparation of alginate oligosaccharides (AOS), that possess special biological activities and is widely used in various fields, such as medicine, food, and chemical industry.
Junjun Yan, Peng Chen, Yan Zeng, Yan Men, Shicheng Mu, Yueming Zhu, Yefu Chen, Yuanxia Sun   +7 more
doaj   +1 more source

Autophagy and mitophagy in pancreatic β‐cell homeostasis and their involvement in diabetes pathophysiology

FEBS Letters, EarlyView.
This review focuses on the role of autophagy and mitophagy in maintaining pancreatic β‐cell function and homeostasis. We discuss how genetic defects affecting these pathways contribute to the development of type 1, type 2, monogenic, and gestational diabetes. We further explore their potential as therapeutic targets. Created in BioRender.
Yunkyeong Lee, Mingming Tong, Anna L. Gloyn   +2 more
wiley   +1 more source

Extracellular enzymes in bioremediation: potential and limitations

, 2016
Bioremediation processes often pivot on the activity of living organisms such as microorganisms, plants, algae. Bioremediation of contaminated soil and water finds very efficient tools in extracellular enzymes.
RAO, MARIA ANTONIETTA
core  

X-ray structure and function studies of key enzymes for biomass conversion [PDF]

, 2013
The need for large enzyme quantities due to the difficult hydrolysis of recalcitrant polysaccharides is still a major barrier to economical biomass conversion for biofuel production.
Wu, Miao
core   +1 more source

BsuMI regulates DNA transformation in Bacillus subtilis besides the defense system and the constructed strain with BsuMI-absence is applicable as a universal transformation platform for wild-type Bacillus

Microbial Cell Factories
Background To effectively introduce plasmids into Bacillus species and conduct genetic manipulations in Bacillus chassis strains, it is essential to optimize transformation methods.
Zhao Xingya, Fu Xiaoping, Zhen Jie, Yang Jun, Zheng Hongchen, Bai Wenqin, Song Hui   +6 more
doaj   +1 more source

N-Terminal Sequences of Signal Peptides Assuming Critical Roles in Expression of Heterologous Proteins in Bacillus subtilis

Microorganisms
The N-terminal sequences of proteins and their corresponding encoding sequences may play crucial roles in the heterologous expression. In this study, the secretory expression of alkaline pectin lyase APL in B.
Meijuan Zhang, Jie Zhen, Jia Teng, Xingya Zhao, Xiaoping Fu, Hui Song, Yeni Zhang, Hongchen Zheng, Wenqin Bai   +8 more
doaj   +1 more source

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source

Ionic Liquids as (Co-)Solvents for Nonhydrolytic Enzymes

, 2012
Summary This chapter contains sections titled: Nomenclature of ILs Ionic Liquids and Nonhydrolytic Enzymes Use of ILs in Oxidoreductase-Catalyzed Enzymatic Reactions ILs in Lyase-Catalyzed Reactions Prospects ...
Daniela Gamenara, Gustavo Seoane, Pablo Domínguez de María, Seoane, Gustavo,, Gamenara, Daniela,, Saenz Mendez, Patricia,, Dominguez de Maria, Pablo,, Patricia Saenz Méndez   +7 more
core   +1 more source