Results 21 to 30 of about 1,893,519 (318)

Structural insight into catalytic mechanism of PET hydrolase

open access: yesNature Communications, 2017
Poly-ethylene terephthalate (PET) is a widely used plastic which accumulates in the environment with detrimental consequences. Here the authors report crystal structures of a PET-hydrolyzing enzyme from the microbe Ideonella sakaiensis bound to substrate
Xu Han   +9 more
doaj   +1 more source

A bioflocculant from Corynebacterium glutamicum and its application in acid mine wastewater treatment

open access: yesFrontiers in Bioengineering and Biotechnology, 2023
Although many microorganisms have been found to produce bioflocculants, and bioflocculants have been considered as attractive alternatives to chemical flocculants in wastewater treatment, there are few reports on bioflocculants from the safe strain C ...
Yinlu Liu   +8 more
doaj   +1 more source

Formation of Enzyme-Enzyme Heteroconjugates

open access: yesAgricultural and Biological Chemistry, 1984
Enzyme-enzyme heteroconjugates of phosphoglyceromutase, enolase and pyruvate kinase, which sequentially act to produce ATP in the glycolytic pathway, were constructed with a bifunctional reagent, N-succinimidyl 3-(2-pyridyldithio)propionate. The conjugates of phospho-glyceromutase-enolase, enolase-pyruvate kinase and phosphoglyceromutase-enolase ...
Koji IKURA   +3 more
openaire   +2 more sources

Purification and Characterization of a Novel Alginate Lyase from the Marine Bacterium Bacillus sp. Alg07

open access: yesMarine Drugs, 2018
Alginate oligosaccharides with different bioactivities can be prepared through the specific degradation of alginate by alginate lyases. Therefore, alginate lyases that can be used to degrade alginate under mild conditions have recently attracted public ...
Peng Chen   +4 more
doaj   +1 more source

Enzyme Kinetics at High Enzyme Concentration

open access: yesBulletin of Mathematical Biology, 2000
We re-visit previous analyses of the classical Michaelis-Menten substrate-enzyme reaction and, with the aid of the reverse quasi-steady-state assumption, we challenge the approximation d[C]/dt approximately 0 for the basic enzyme reaction at high enzyme concentration.
Schnell, S, Maini, P
openaire   +3 more sources

In vitro simulated study of macronutrient digestion in complex food using digestive enzyme supplement

open access: yesHeliyon
Digestive enzymes secreted by the body are vital for digestion and nutrient absorption. Enzyme supplements are commonly used to support them in achieving optimal digestion.
Abhijit Rathi   +4 more
doaj   +1 more source

Biophysical characterization of the structural stability of Helix lucorum hemocyanin

open access: yesBiotechnology & Biotechnological Equipment, 2021
The structural stability of the hеmocyanin purified from the hеmolymph of gаrden snаils Helix lucorum (HlH) was investigated by means of far-UV circular dichroism (CD), differential scanning calorimetry (DSC) and transmission electron microscopy (TEM ...
Krassimira Idakieva   +5 more
doaj   +1 more source

Glycosyltransferase engineering and multi-glycosylation routes development facilitating synthesis of high-intensity sweetener mogrosides

open access: yesiScience, 2022
Summary: Mogrosides are widely served as natural zero-calorie sweeteners. To date, the biosynthesis of high-intensity sweetness mogrosides V from mogrol has not been achieved because of inefficient and uncontrollable multi-glycosylation process.
Jiao Li   +8 more
doaj   +1 more source

High copy number and highly stable Escherichia coli–Bacillus subtilis shuttle plasmids based on pWB980

open access: yesMicrobial Cell Factories, 2020
Background pWB980 derived from pUB110 is a promising expression vector in Bacillus for its high copy number and high stability. However, the low transformation rate of recombinant plasmids to the wild cells limited the application of it.
XingYa Zhao   +8 more
doaj   +1 more source

DELP Treatment on Vision and Retinal Microcirculation in Patients With Acute Ischemic Stroke: Report of Five Cases and Literature Review

open access: yesTherapeutic Apheresis and Dialysis, EarlyView.
ABSTRACT Background The delipid extracorporeal lipoprotein filter from plasma (DELP) treatment can effectively reduce blood lipid, increase blood flow, and improve neurological deficits in patients with acute ischemic stroke (AIS). However, its effect on vision and retinal microcirculation in stroke patients has never been reported.
Ning Li   +9 more
wiley   +1 more source

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